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Volume 64, Issue Pt_4

sp. nov., isolated from a deep-sea sediment Free

Abstract

A moderately halophilic bacterial strain, designated XH204, was isolated from deep-sea sediment core (45° 58′ S 163° 11′ W) of the South Pacific Gyre during the Integrated Ocean Drilling Program Expedition 329. The strain was Gram-stain-positive, rod-shaped, motile by peritrichous flagella and produced ellipsoidal endospores subterminally positioned within swollen sporangia. Growth of strain XH204 occurred at 15–42 °C (optimum 37 °C), in the presence of 0–14 % (w/v) NaCl (optimum 4 %) and at pH 7.0–10.0 (optimum pH 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH204 belonged to the genus and showed the highest sequence similarity to CL-MP28 (95.6 %); strain XH204 exhibited 93.4 % 16S rRNA gene sequence similarity to the type strain of the type species of the genus , HTE831. The dominant fatty acids of strain XH204 were anteiso-C, iso-C and iso-C. The cell wall of strain XH204 contained -diaminopimelic acid as the diagnostic diamino acid, and ribose, glucose and galactose as the major whole-cell sugars. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Menaquinone-7 (MK-7) was the only isoprenoid quinone and the DNA G+C content was 38.8 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic analysis, strain XH204 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XH204 ( = DSM 25873 = JCM 18381).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41276141)
  • National High Technology Research & Development Program of China (Award 2012AA091605)
  • China Ocean Mineral Resources Research & Development Association (Award DY125-15-R-03)
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2014-04-01
2024-04-23
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Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment
Int J Syst Evol Microbiol 64, 1278 (2014); https://doi.org/10.1099/ijs.0.056481-0
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