RT Journal Article SR Electronic(1) A1 Zhong, Zhi-Ping A1 Liu, Ying A1 Liu, Liang-Zi A1 Wang, Fang A1 Zhou, Yu-Guang A1 Liu, Zhi-PeiYR 2014 T1 Rheinheimera tuosuensis sp. nov., isolated from a saline lake JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_4 SP 1142 OP 1148 DO https://doi.org/10.1099/ijs.0.056473-0 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-negative bacterium, strain TS-T4T, was isolated from Tuosu Lake, a saline lake (salinity 5.4 %, w/w) in Qaidam basin, Qinghai, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain TS-T4T were non-spore-forming rods, 0.4–0.8 µm wide and 1.7–2.3 µm long, and motile by means of a single polar flagellum. Strain TS-T4T was strictly heterotrophic, aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–7.0 % (w/v) NaCl (optimum, 3.0–4.0 %) and at 4–40 °C (optimum, 30–35 °C) and pH 7.0–10.5 (optimum, pH 8.5–9.0). Strain TS-T4T contained Q-8 as the sole respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids, as for other members of the genus Rheinheimera . The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C17 : 1ω8c and C18 : 1ω7c. The DNA G+C content was 50.2 mol% (T m). Phylogenetic trees based on sequences of the 16S rRNA gene and a conserved portion of the gyrase B gene (gyrB) showed that strain TS-T4T was associated with the genus Rheinheimera ; the strain showed the highest 16S rRNA gene sequence similarity to Rheinheimera longhuensis LH2-2T (97.1 %) and then to Rheinheimera pacifica KMM 1406T (97.0 %). DNA–DNA relatedness of strain TS-T4T with R. longhuensis LH2-2T and R. pacifica NBRC 103167 was 53±2.5 and 48±2 %, respectively. Based on the data presented, it is concluded that TS-T4T represents a novel species of the genus Rheinheimera , for which the name Rheinheimera tuosuensis sp. nov. is proposed. The type strain is TS-T4T ( = CGMCC 1.12461T = JCM 19264T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056473-0