1887

Abstract

A Gram-staining-negative bacterium, strain TS-T4, was isolated from Tuosu Lake, a saline lake (salinity 5.4 %, w/w) in Qaidam basin, Qinghai, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain TS-T4 were non-spore-forming rods, 0.4–0.8 µm wide and 1.7–2.3 µm long, and motile by means of a single polar flagellum. Strain TS-T4 was strictly heterotrophic, aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–7.0 % (w/v) NaCl (optimum, 3.0–4.0 %) and at 4–40 °C (optimum, 30–35 °C) and pH 7.0–10.5 (optimum, pH 8.5–9.0). Strain TS-T4 contained Q-8 as the sole respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids, as for other members of the genus . The predominant fatty acids (>10 %) were summed feature 3 (Cω7 and/or Cω6), C, Cω8 and Cω7. The DNA G+C content was 50.2 mol% ( ). Phylogenetic trees based on sequences of the 16S rRNA gene and a conserved portion of the gyrase B gene () showed that strain TS-T4 was associated with the genus ; the strain showed the highest 16S rRNA gene sequence similarity to LH2-2 (97.1 %) and then to KMM 1406 (97.0 %). DNA–DNA relatedness of strain TS-T4 with LH2-2 and NBRC 103167 was 53±2.5 and 48±2 %, respectively. Based on the data presented, it is concluded that TS-T4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TS-T4 ( = CGMCC 1.12461 = JCM 19264).

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2014-04-01
2019-12-09
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