1887

Abstract

Four Gram-staining-negative, catalase- and oxidase-positive, pale-orange pigmented bacterial strains (435-08, 47B-3-09, 412R-09 and 60B-3-09) were isolated from diseased rainbow trout. Analysis of their 16S rRNA gene sequences suggested their adscription to the genus . Strains formed two phylogenetic groups represented by strains 435-08 and 47B-3-09 (group A), and strains 412R-09 and 60B-3-09 (group B) displaying 16S rRNA sequence similarities greater than 99.8–99.9 % within their respective groups. Strain 435-08 exhibited the highest levels of similarity with WB-1.1.56 (98.6 % sequence similarity) and strain 412R-09 with KUC-1 and WB-1.1.56 (98.9 % and 98.6 % sequence similarity, respectively). DNA–DNA hybridization studies showed low levels of relatedness between strain 435-08 and strain 412R-09 and between both strains and the most closely related species of the genus . The genomic DNA G+C contents of strains 435-08 and 412R-09 were 36.2 and 34.3 mol%, respectively. The predominant respiratory quinone of both strains was MK-6 and the major fatty acids were iso-C, Cω7 and C. The two groups of strains could be distinguished from each other and from related species of the genus by a number of phenotypic properties. Phylogenetic, genotypic and phenotypic evidence indicated that strains of groups A and B represent two novel species of the genus , for which the names sp. nov. (type strain 435-08 = CECT 7791 = CCUG 60100) and sp. nov. (type strain 412R-09 = CECT 7911 = CCUG 60099) are proposed.

Funding
This study was supported by the:
  • , Spanish Office for Science and Technology (CDETI) , (Award CENIT 2007-2010 (ACUISOST))
  • , Spanish Ministry of Science and Innovation , (Award CGL2010-19303)
  • , Junta de Andalucía , (Award P10-CVI-6226)
  • , Västra Götaland Region , (Award VGFOUREG-157801, VGFOUREG-83080 and VGFOUREG-30781)
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2014-02-01
2020-05-26
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