1887

Abstract

Two strains (J3-AN59 and J3-N84) of Gram-stain-negative, aerobic and rod-shaped bacteria were isolated from the roots of fresh rice plants. The 16S rRNA gene sequence similarity results showed that the similarity between strains J3-AN59 and J3-N84 was 100 %. Both strains were phylogenetically related to members of the genus , and they were most closely related to ACCC 06128 (97.43 %). Similarities in the sequences of housekeeping genes between strains J3-AN59 and J3-N84 and those of recognized species of the genus were less than 90 %. The polar lipid profiles of both strains were predominantly composed of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid. The major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6) and C. The DNA G+C contents of J3-AN59 and J3-N84 were 55.7 and 57.1 mol%, respectively. The DNA–DNA relatedness value between J3-AN59 and J3-N84 was 89 %, and strain J3-AN59 showed 9 % DNA–DNA relatedness to ACCC 06128, the most closely related strain. Based on this evidence, we found that J3-AN59 and J3-N84 represent a novel species in the genus and we propose the name sp. nov. The type strain is J3-AN59 ( = ACCC 05916 = KCTC 23652).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 41271273)
  • , National High Technology Research and Development Program (863) of China , (Award 2013AA102802)
  • , Special Scientific Research Funds for Commonwealth Agricultural Industry , (Award 201103005-01)
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2014-04-01
2021-02-26
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