Kordiaaquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia
A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301T was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301T predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412T (95.0 %), K. algicida KCTC 8814PT (94.4 %) and K. antarctica IMCC3317T (94.2 %), and formed a phylogenetic lineage tightly associated with species of the genus Kordia. The polar lipid profile of strain CC-AMZ-301T consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5 % of total) were iso-C17 : 0 3-OH, iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301T is proposed to represent a novel species of the genus Kordia, for which the name Kordiaaquimaris sp. nov. is proposed; the type strain is CC-AMZ-301T ( = JCM 18556T = BCRC 80464T). An emended description of the genus Kordia is also proposed.
BarrowG. I.,
FelthamR. K. A.(editors) (1993).Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
BernardetJ. F.,
NakagawaY.,
HolmesB.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol52, 1049–1070. [View Article][PubMed]
ChoiA.,
OhH.-M.,
YangS.-J.,
ChoJ.-C.(2011).Kordia periserrulae sp. nov., isolated from a marine polychaete Periserrula leucophryna, and emended description of the genus Kordia
. . Int J Syst Evol Microbiol61, 864–869. [View Article][PubMed]
EmbleyT. M.,
WaitR.(1994). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by
GoodfellowM.,
O’DonnellA. G.
. Chichester:: Wiley;.
HameedA.,
ShahinaM.,
LinS. Y.,
SridharK. R.,
YoungL. S.,
LeeM. R.,
ChenW. M.,
ChouJ. H.,
YoungC. C.(2012).Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan. . FEMS Microbiol Lett333, 37–45. [View Article][PubMed]
HeinerC. R.,
HunkapillerK. L.,
ChenS. M.,
GlassJ. I.,
ChenE. Y.(1998). Sequencing multimegabase-template DNA with BigDye terminator chemistry. . Genome Res8, 557–561.[PubMed]
KämpferP.,
KroppenstedtR. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol42, 989–1005. [View Article]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
MurrayR. G. E.,
DoetschR. N.,
RobinowC. F.(1994). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
ReichenbachH.(1992). The order Cytophagales
. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 3631–3675. Edited by
BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK.-H.
. New York:: Springer;. [CrossRef]
SmibertR. M.,
KriegN. R.(1994). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
TeatherR. M.,
WoodP. J.(1982). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol43, 777–780.[PubMed]
TindallB. J.,
Rosselló-MóraR.,
BusseH.-J.,
LudwigW.,
KämpferP.(2010). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol60, 249–266. [View Article][PubMed]
Kordiaaquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia