1887

Abstract

A novel facultatively anaerobic bacterium, designated strain LAM0618, was isolated from biogas slurry samples collected from the large-scale anaerobic digester of Modern Farming Corporation in Hebei Province, China. Cells of strain LAM0618 were Gram-stain-positive, motile, non-spore-forming and short-rod-shaped. The optimal temperature and pH for growth were 30 °C and 7.0, respectively. The strain did not require NaCl for growth but tolerated up to 70 g NaCl l. The major fatty acids of strain LAM0618 were iso-C, anteiso-C, iso-C, C and C. The predominant menaquinones of strain LAM0618 were menaquinone 7 (MK-7) and menaquinone 6 (MK-6). The main polar lipids of strain LAM0618 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and six unknown glycolipids. The genomic DNA G+C content was 41 mol% as determined by the method. Analysis of the 16S rRNA gene sequence revealed that strain LAM0618 was a member of the genus , and was most closely related to ‘ ’ DSM 24639, DSM 20580, DSM 20636 and DSM 4747, with 96.9, 95.7, 95.6 and 94.9 % sequence similarity, respectively. Based on its phenotypic and genotypic properties, strain LAM0618 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAM0618 ( = ACCC 06121 = JCM 19187).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31270159)
  • , National Key Technology R&D Program of China , (Award 2011BAD11B05 and 2013BAD05B04F02)
  • , National Nonprofit Institute Research Grant of CAAS , (Award 202-37)
  • , Science Foundation of Modern Farming Group , (Award MF20100518)
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2014-02-01
2020-06-01
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