1887

Abstract

A Gram-staining-positive, aerobic, motile, endospore-forming, rod-shaped bacterium, designated GD04, was isolated from a saline soil sample taken in southern China and was characterized by means of a polyphasic approach. Growth occurred with 0.5–12 % (w/v) NaCl (optimum 1–2 %) and at pH 7.0–9.5 (optimum pH 8.0) and 10–45 °C (optimum 30 °C). According to the results of a phylogenetic analysis, strain GD04 belonged to the genus , and was related most closely to type strains of the species and (96.5 and 96.5 % 16S rRNA gene sequence similarities, respectively). The peptidoglycan amino acid type was A4β, containing -ornithine and -aspartic acid. The major respiratory quinone was menaquinone-7 (MK-7). The polar lipid profile of strain GD04 contained predominantly diphosphatidylglycerol with moderate amounts of phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown lipid, and a minor amount of another unknown lipid. The G+C content of genomic DNA was 39.3 mol%. The dominant cellular fatty acids were iso-C, iso-C and anteiso-C. The phenotypic, chemotaxonomic, phylogenetic and genotypic data indicated that strain GD04 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GD04 ( = KCTC 33116 = CGMCC 1.12408).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 41201227)
  • , Team Project of Guangdong Natural Science Foundation , (Award S2012030006144 and S2011030002882)
  • , China Postdoctoral Science Foundation Grant , (Award 2013M531828)
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2014-05-01
2020-07-03
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