1887

Abstract

A novel yeast species was found repeatedly and in high cell densities in underground-nesting stingless bees of the species and their provisions in northern Minas Gerais (Brazil). One additional strain was isolated from bee-collected pollen in Cuba. Phylogenetic analyses based on rRNA gene sequences (D1/D2 large subunit gene and internal transcribed spacer) indicated that the novel species belongs to the clade and is most closely related to (iter. nom. ) . Growth reactions on carbon and nitrogen sources were typical of those observed in related species of the clade. PCR-fingerprinting with mini- and microsatellite specific primers allowed the distinction of the novel species from , and a yet undescribed species represented by strain CBS 4353. On the basis of phylogenetic relationships, the novel species is assigned to the genus despite the failure to observe sexual reproduction after extensive mating tests. We propose the name f. a., sp. nov. (Mycobank MB 804285) and designate UFMG PST 09 ( = MUCL 53320 = CBS 12811) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055897-0
2013-10-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/10/3896.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055897-0&mimeType=html&fmt=ahah

References

  1. Absy M. L., Camargo J. M. F., Kerr W. E., Miranda I. P. A.. ( 1984;). Espécies de plantas visitadas por Meliponinae (Hymenoptera: Apoidea), para coleta de pólen na região do médio Amazonas. . Rev Bras Biol 44:, 227–237.
    [Google Scholar]
  2. Clement M., Posada D., Crandall K. A.. ( 2000;). tcs: a computer program to estimate gene genealogies. . Mol Ecol 9:, 1657–1659. [CrossRef][PubMed]
    [Google Scholar]
  3. Daniel H. M., Vrancken G., Takrama J. F., Camu N., De Vos P., De Vuyst L.. ( 2009;). Yeast diversity of Ghanaian cocoa bean heap fermentations. . FEMS Yeast Res 9:, 774–783. [CrossRef][PubMed]
    [Google Scholar]
  4. Fidalgo-Jiménez A., Daniel H. M., Evrard P., Decock C., Lachance M. A.. ( 2008;). Metschnikowia cubensis sp. nov., a yeast species isolated from flowers in Cuba. . Int J Syst Evol Microbiol 58:, 2955–2961. [CrossRef][PubMed]
    [Google Scholar]
  5. Lachance M. A., Kurtzman C. P.. ( 2013;). The yeast genus Tortispora gen. nov., description of Tortispora ganteri sp. nov., Tortispora mauiana f.a., sp. nov., Tortispora agaves f.a., sp. nov., Tortispora sangerardonensis f.a., sp. nov., Tortispora cuajiniquilana f.a., sp. nov., Tortispora starmeri f.a., sp. nov. and Tortispora phaffii f.a., sp. nov., reassignment of Candida caseinolytica to Tortispora caseinolytica f.a., comb. nov., emendation of Botryozyma, and assignment of Botryozyma, Tortispora gen. nov. and Trigonopsis to the family Trigonopsidaceae fam. nov. . Int J Syst Evol Microbiol 63:, 3104–3114. [CrossRef][PubMed]
    [Google Scholar]
  6. Lachance M. A., Dobson J., Wijayanayaka D. N., Smith A. M. E.. ( 2010;). The use of parsimony network analysis for the formal delineation of phylogenetic species of yeasts: Candida apicola, Candida azyma, and Candida parazyma sp. nov., cosmopolitan yeasts associated with floricolous insects. . Antonie van Leeuwenhoek 97:, 155–170. [CrossRef][PubMed]
    [Google Scholar]
  7. Lima-Verde L. W., Freitas B. M.. ( 2002;). Occurrence and biogeographic aspects of Melipona quinquefasciata in NE Brazil (Hymenoptera, Apidae). . Braz J Biol 62:, 479–486. [CrossRef][PubMed]
    [Google Scholar]
  8. McNeill J., Barrie F. R., Buck W. R., Demoulin V., Greuter W., Hawksworth D. L., Herendeen P. S., Knapp F. S., Makhold K.. & other authors ( 2012;). International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code). Regnum Vegetabile 154. Koenigstein:: Koeltz Scientific Books;.
    [Google Scholar]
  9. Nogueira-Neto P.. ( 1997;). Vida e Criação de Abelhas Indígenas Sem Ferrão. São Paulo:: Editora Nogueirapis;.
    [Google Scholar]
  10. Pereira J. O. P.. ( 2006;). Diversidade genética da abelha sem ferrão Melipona quinquefasciata baseada no seqüenciamento das regiões ITS1 parcial e 18S do DNA ribossômico nuclear. Departamento de Zootecnia, Universidade Federal do Ceará;, Brazil:. Doctoral thesis.
    [Google Scholar]
  11. Robert V., Evrard P., Hennebert G. L.. ( 1997;). BCCM/ALLEV 2.00 an automated system for the identification of yeasts. . Mycotaxon 64:, 455–463.
    [Google Scholar]
  12. Rosa C. A., Viana E. M., Martins R. P., Antonini Y., Lachance M. A.. ( 1999;). Candida batistae, a new yeast species associated with solitary digger nesting bees in Brazil. . Mycologia 91:, 428–433. [CrossRef]
    [Google Scholar]
  13. Rosa C. A., Lachance M. A., Silva J. O., Teixeira A. C. P., Marini M. M., Antonini Y., Martins R. P.. ( 2003;). Yeast communities associated with stingless bees. . FEMS Yeast Res 4:, 271–275. [CrossRef][PubMed]
    [Google Scholar]
  14. São-Thiago P. S., Bastos E. M. A. F., Sabino W. O., Silveira M., Antonini Y.. ( 2010;). Ecologia da nidificação de Mandaçaia-do-chão (M. quinquefasciata) no Parque Estadual Veredas do Peruaçu. . MG Biota 3:, 4–14.
    [Google Scholar]
  15. Tamura K.. ( 1992;). Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. . Mol Biol Evol 9:, 678–687.[PubMed]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055897-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055897-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error