1887

Abstract

A novel actinomycete, designated strain NEAU-ycm2, was isolated from edible Chinese black ants ( Roger) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus . The 16S rRNA gene sequence of strain NEAU-ycm2 showed highest similarity to those of 274745 (99.12 %), TJ2-2 (98.85 %), GUI 15 (98.76 %), TT 97-42 (98.42 %) and LK2-10 (98.21 %). Phylogenetic analysis based on the 16S rRNA gene and gene demonstrated that strain NEAU-ycm2 is a member of the genus and supported the close phylogenetic relationship to 274745, JCM 12833 and GUI 15. Furthermore, a combination of DNA–DNA hybridization and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. Therefore, it is proposed that NEAU-ycm2 represents a novel species of the genus of , for which the name sp. nov. is proposed. The type strain is NEAU-ycm2 ( = CGMCC 4.7100 = DSM 45886).

Funding
This study was supported by the:
  • , National Key Project for Basic Research , (Award 2010CB126102)
  • , National Natural Science Foundation of China , (Award 30771427 and 30971937)
  • , National Outstanding Youth Foundation , (Award 31225024)
  • , Special Foundation for Scientific and Technological Innovation Research of Harbin , (Award 2011RFXXN038)
  • , Natural Science Foundation of Heilongjiang Province , (Award C201029)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055863-0
2014-02-01
2020-07-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/495.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055863-0&mimeType=html&fmt=ahah

References

  1. Buchner P. ( 1965 ). Endosymbiosis of Animals With Plant Microorganisms. New York:: Interscience Publishers;.
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267284. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  6. Garcia L. C., Martínez-Molina E., Trujillo M. E. ( 2010 ). Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . . Int J Syst Evol Microbiol 60, 331337. [CrossRef] [PubMed]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [CrossRef]
    [Google Scholar]
  8. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501509. [CrossRef]
    [Google Scholar]
  9. Huß V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  11. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. ( 2000 ). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. . Int J Syst Evol Microbiol 50, 20312036. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kroiss J., Kaltenpoth M., Schneider B., Schwinger M. G., Hertweck C., Maddula R. K., Strohm E., Svatoš A. ( 2010 ). Symbiotic Streptomycetes provide antibiotic combination prophylaxis for wasp offspring. . Nat Chem Biol 6, 261263. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lechevalier H. A., Lechevalier M. P. ( 1970a ). A critical evaluation of the genera of aerobic actinomycetes. . In The Actinomycetes, pp. 393405. Edited by Prauser H. . Jena:: Gustav Fischer Verlag;.
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. ( 1970b ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435443. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, (Special Publication no. 6), pp. 227291. Edited by Dietz A., Thayer D. W. . Arlington, VA:: Society of Industrial Microbiology;.
    [Google Scholar]
  18. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977 ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5, 249260. [CrossRef]
    [Google Scholar]
  19. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206. [CrossRef]
    [Google Scholar]
  20. May R. M. ( 1988 ). How many species are there on Earth?. Science 241, 14411449. [CrossRef] [PubMed]
    [Google Scholar]
  21. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178182. [CrossRef] [PubMed]
    [Google Scholar]
  22. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  24. Oh D. C., Poulsen M., Currie C. R., Clardy J. ( 2009 ). Dentigerumycin: a bacterial mediator of an ant–fungus symbiosis. . Nat Chem Biol 5, 391393. [CrossRef] [PubMed]
    [Google Scholar]
  25. Poulsen M., Oh D. C., Clardy J., Currie C. R. ( 2011 ). Chemical analyses of wasp-associated Streptomyces bacteria reveal a prolific potential for natural products discovery. . PLoS ONE 6, e16763. [CrossRef] [PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  27. Scott J. J., Oh D. C., Yuceer M. C., Klepzig K. D., Clardy J., Currie C. R. ( 2008 ). Bacterial protection of beetle-fungus mutualism. . Science 322, 63. [CrossRef] [PubMed]
    [Google Scholar]
  28. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [CrossRef]
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  31. Waksman S. A. ( 1961 ). Classification, Identification and Descriptions of Genera and Species, vol. 2. The Actinomycetes. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  32. Waksman S. A. ( 1967 ). The Actinomycetes. A Summary of Knowledge. New York:: Ronald;.
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  34. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176178. [CrossRef]
    [Google Scholar]
  35. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y. ( 2011 ). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61, 11651169. [CrossRef] [PubMed]
    [Google Scholar]
  36. Yokota A., Tamura T., Hasegawa T., Huang L. H. ( 1993 ). Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 43, 805812. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055863-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055863-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error