1887

Abstract

A novel thermotolerant, anaerobic, Gram-stain-positive, spore-forming bacterium was isolated from a hydrothermal chimney in Prony Bay, New Caledonia. This strain, designated FatNI3, grew at 15–55 °C (optimum 30 °C) and at pH 5.8–8.9 (optimum 7.7). It was slightly halophilic, requiring at least 0.5 % NaCl for growth (optimum 2.5–3.0 %), and was able to grow at up to 6 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Growth of strain FatNI3 was inhibited in the presence of sulfite (2 mM) or nitrite (2 mM). Strain FatNI3 fermented cellobiose, glucose, mannose, maltose, sucrose, galactose, lactose, ribose, fructose, rhamnose, raffinose, xylose, yeast extract, peptone and biotrypticase. The main fermentation products from glucose metabolism were acetate, ethanol, H and CO. The predominant cellular fatty acids were iso-C and anteiso-C. The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and unknown glycolipids and phospholipids. The G+C content of the genomic DNA was 36.6 mol%. On the basis of phylogenetic and physiological properties, strain FatNI3 ( = DSM 25904 = JCM 18391) belonging to the phylum , class , order , is proposed as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055756-0
2014-04-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1160.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055756-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. ( 1979 ). Methanogens: reevaluation of a unique biological group. . Microbiol Rev 43, 260296.[PubMed]
    [Google Scholar]
  3. Brazelton W. J., Schrenk M. O., Kelley D. S., Baross J. A. ( 2006 ). Methane- and sulfur-metabolizing microbial communities dominate the Lost City hydrothermal field ecosystem. . Appl Environ Microbiol 72, 62576270. [View Article] [PubMed]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  5. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. ( 1994 ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44, 812826. [View Article] [PubMed]
    [Google Scholar]
  6. Cord-Ruwisch R. ( 1985 ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. . J Microbio Methods 4, 3336. [View Article] [PubMed]
    [Google Scholar]
  7. Edgar R. C. ( 2004 ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32, 17921797. [View Article] [PubMed]
    [Google Scholar]
  8. Erauso G., Postec A., Quéméneur M., Ben Aïssa F., Hamelin J., Ménez B., Gérard E., Gérard M., Pelletier B. & other authors ( 2012 ). Microbial communities associated with alkaline hydrothermal systems of Prony Bay, New Caledonia. . In 14th International Symposium on Microbial Ecology, The Power of the Small, poster 093A, p. 160. Copenhagen, Denmark:, August 2012.
    [Google Scholar]
  9. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. ( 1997 ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. . Int J Syst Bacteriol 47, 10131019. [View Article] [PubMed]
    [Google Scholar]
  10. Fardeau M.-L., Magot M., Patel B. K. C., Thomas P., Garcia J.-L., Ollivier B. ( 2000 ). Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. . Int J Syst Evol Microbiol 50, 21412149. [View Article] [PubMed]
    [Google Scholar]
  11. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  12. Hungate R. E. ( 1969 ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3B, 117132. [View Article]
    [Google Scholar]
  13. Jabari L., Gannoun H., Cayol J.-L., Hamdi M., Fauque G., Ollivier B., Fardeau M.-L. ( 2012 ). Characterization of Defluviitalea saccharophila gen. nov., sp. nov., a thermophilic bacterium isolated from an upflow anaerobic filter treating abattoir wastewaters, and proposal of Defluviitaleaceae fam. nov.. Int J Syst Evol Microbiol 62, 550555. [View Article] [PubMed]
    [Google Scholar]
  14. Khelifi N., Ben Romdhane E., Hedi A., Postec A., Fardeau M.-L., Hamdi M., Tholozan J.-L., Ollivier B., Hirschler-Réa A. ( 2010 ). Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. . Extremophiles 14, 297304. [View Article] [PubMed]
    [Google Scholar]
  15. Kuykendall L. D., Roy M. A., O’Neil J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  16. Lakhal R., Pradel N., Postec A., Hamdi M., Ollivier B., Godfroy A., Fardeau M. L. ( 2013 ). Vallitalea guaymasensis gen. nov., sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 63, 30193023. [View Article] [PubMed]
    [Google Scholar]
  17. Launay J., Fontes J. C. ( 1985 ). Les sources thermales de Prony (Nouvelle-Calédonie) et leurs précipités chimiques. Exemple de formation de brucite primaire. . Geol Fr 1, 83100.
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  19. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  20. Miller T. L., Wolin M. J. ( 1974 ). A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. . Appl Microbiol 27, 985987.[PubMed]
    [Google Scholar]
  21. Pelletier B., Chevillon C., Menou J. L., Butscher J., Folcher E., Geoffray C., Bore J. M., Panche J. Y., Perrier J. ( 2006 ). Plongées, forage et cartographie Baie du Prony et Banc Gail, lagon Sud de Nouvelle-Calédonie, campagne 2005-NC-PL du N.O. ALIS 13–17 June 2005 et cartographie baie du Prony et canal Woodin N.O. ALIS 25–26 September 2005. . In Missions Sci. Terre, Géol-Géophys vol. 70. Nouméa, New Caledonia:: Nouméa IRD;.
    [Google Scholar]
  22. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20, 16.
    [Google Scholar]
  23. Schrenk M. O., Kelley D. S., Bolton S. A., Baross J. A. ( 2004 ). Low archaeal diversity linked to subseafloor geochemical processes at the Lost City Hydrothermal Field, Mid-Atlantic Ridge. . Environ Microbiol 6, 10861095. [View Article] [PubMed]
    [Google Scholar]
  24. Sorokin D. Y., Tourova T. P., Panteleeva A. N., Kaparullina E. N., Muyzer G. ( 2012 ). Anaerobic utilization of pectinous substrates at extremely haloalkaline conditions by Natranaerovirga pectinivora gen. nov., sp. nov., and Natranaerovirga hydrolytica sp. nov., isolated from hypersaline soda lakes. . Extremophiles 16, 307315. [View Article] [PubMed]
    [Google Scholar]
  25. Tamura K., Nei M., Kumar S. ( 2004 ). Prospects for inferring very large phylogenies by using the neighbor-joining method. . Proc Natl Acad Sci U S A 101, 1103011035. [View Article] [PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.055756-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055756-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error