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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_5

gen. nov., sp. nov., a moderately thermotolerant member of the family Free

Abstract

Two Gram-staining-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains GD05 and GD051, were isolated from a tropical forest soil and a hot spring sediment, respectively. Cells of both strains were facultatively anaerobic, catalase- and oxidase-positive, and could grow optimally at 50 °C, pH 8.0 and with 1 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that these two isolates belonged to the family , but did not show sequence similarities of more than 95 % to members of other related genera. The G+C content of the genomic DNA was 43.7–44.1 mol%. The major cellular fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major menaquinone was MK-7. The peptidoglycan type was A1γ (-diaminopimelic acid direct). On the basis of this polyphasic taxonomic analysis, the novel strains represent a novel species of a new genus in the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain is GD05 ( = CCTCC AB 2013105 = KCTC 33117).

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Funding
This study was supported by the:
  • Guangdong Natural Science Foundation (Award S2012030006114 and S2011030002882)
  • National Key Technology R&D Program of China (Award 2012BAD14B16)
© 2014 IUMS
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2014-05-01
2025-03-17
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Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae
Int J Syst Evol Microbiol 64, 1647 (2014); https://doi.org/10.1099/ijs.0.055608-0
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