RT Journal Article SR Electronic(1) A1 Li, Weiguang A1 Zhang, Duoying A1 Huang, Xiaofei A1 Qin, WenYR 2014 T1 Acinetobacter harbinensis sp. nov., isolated from river water JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_5 SP 1507 OP 1513 DO https://doi.org/10.1099/ijs.0.055251-0 PB Microbiology Society, SN 1466-5034, AB A bacterial strain, HITLi 7T, with nitrifying ability was isolated from the surface water of the Songhua River in China. Cells were Gram-stain-negative, strictly aerobic, oxidase-negative, non-motile coccobacilli, capable of growth in mineral media with acetate as the sole carbon source and ammonia as the sole source of nitrogen. The cells did not grow at 37 °C, but did grow at 2 °C. The DNA G+C content was 45.5 mol%. Results of 16S rRNA gene sequence analysis indicated a close relationship between this isolate and Acinetobacter lwoffii (98.4 % similarity for strain DSM 2403T). rpoB and gyrB gene sequences did not show significant similarity with those from other species of the genus Acinetobacter . Predominant cellular fatty acids were 9-octadecenoic acid (C18 : 1ω9c) and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Acid was not produced from d-glucose, and gelatin was not hydrolysed by the isolate. Genotypic, phenotypic and chemotaxonomic data from this study indicate that the isolate should be classified as a representative of a novel species of the genus Acinetobacter . The name Acinetobacter harbinensis sp. nov. is proposed for the novel species, with HITLi 7T ( = CGMCC 1.12528T = KCTC 32411T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.055251-0