1887

Abstract

A bacterial strain, HITLi 7, with nitrifying ability was isolated from the surface water of the Songhua River in China. Cells were Gram-stain-negative, strictly aerobic, oxidase-negative, non-motile coccobacilli, capable of growth in mineral media with acetate as the sole carbon source and ammonia as the sole source of nitrogen. The cells did not grow at 37 °C, but did grow at 2 °C. The DNA G+C content was 45.5 mol%. Results of 16S rRNA gene sequence analysis indicated a close relationship between this isolate and (98.4 % similarity for strain DSM 2403). B and B gene sequences did not show significant similarity with those from other species of the genus . Predominant cellular fatty acids were 9-octadecenoic acid (Cω9) and summed feature 4 (iso-C 2-OH and/or Cω7). Acid was not produced from -glucose, and gelatin was not hydrolysed by the isolate. Genotypic, phenotypic and chemotaxonomic data from this study indicate that the isolate should be classified as a representative of a novel species of the genus . The name sp. nov. is proposed for the novel species, with HITLi 7 ( = CGMCC 1.12528 = KCTC 32411) as the type strain.

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2014-05-01
2019-10-23
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