1887

Abstract

Four strains of actinomycete, designated strains JR-19, JR-12, JR-29 and JR-41 were isolated from bamboo () rhizosphere soil. Phylogenetic, morphological, chemotaxonomic and phenotypic analysis demonstrated that the four strains belong to the genus . Microscopic observation revealed that the four strains produced spore chains with spiny surfaces. The cell-wall peptidoglycan of the four strains contained -diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9 (H) and MK-9 (H). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that these strains and the members of the genus exhibited moderately high 16S rRNA gene sequence similarities of 98.3–99.3 %, with the most closely related strains being 172115 and JR-43. Based on the phenotypic and genotypic data, the four strains are considered to represent two novel species of the genus , for which the names sp. nov. [to accommodate strains JR-19 (type strain;  = KACC 16472 = NBRC 108883), JR-12 ( = KACC 16471) and JR-29 ( = KACC 16473)] and sp. nov. [for strain JR-41 ( = KACC 16580 = NBRC 108885)] are proposed.

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2014-05-01
2019-10-22
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References

  1. Bérdy J. . ( 2005; ). Bioactive microbial metabolites. . J Antibiot (Tokyo) 58:, 1–26. (Tokyo). [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  3. Cui X. L. , Mao P. H. , Zeng M. , Li W. J. , Zhang L. P. , Xu L. H. , Jiang C. L. . ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  4. Euzéby J. P. . ( 2012; ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . [Last full update 3 June 2012]. http://www.bacterio.cict.fr/.
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Hu H. , Lin H. P. , Xie Q. , Li L. , Xie X. Q. , Sun M. , Hong K. . ( 2011; ). Streptomyces shenzhenensis sp. nov., a novel actinomycete isolated from mangrove sediment. . Antonie van Leeuwenhoek 100:, 631–637. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Küster E. , Williams S. T. . ( 1964; ). Selection of media for isolation of Streptomycetes . . Nature 202:, 928–929. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lanoot B. , Vancanneyt M. , Hoste B. , Vandemeulebroecke K. , Cnockaert M. C. , Dawyndt P. , Liu Z. , Huang Y. , Swings J. . ( 2005; ). Grouping of streptomycetes using 16S-ITS RFLP fingerprinting. . Res Microbiol 156:, 755–762. [CrossRef] [PubMed]
    [Google Scholar]
  14. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lazzarini A. , Cavaletti L. , Toppo G. , Marinelli F. . ( 2000; ). Rare genera of actinomycetes as potential producers of new antibiotics. . Antonie van Leeuwenhoek 78:, 399–405. [CrossRef] [PubMed]
    [Google Scholar]
  16. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P. , Lechevalier H. A. . ( 1980; ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Society for Industrial Microbiology Special Publication no. 6), pp. 227–291. Edited by Dietz A. , Thayer D. W. . . Fairfax, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  18. Lee H. J. , Whang K. S. . ( 2010; ). Biodiversity and phylogenetic analysis of Streptomyces collected from bamboo forest soil. . Kor J Microbiol 46:, 262–269.
    [Google Scholar]
  19. Lee H. J. , Han S. I. , Whang K. S. . ( 2012; ). Streptomyces gramineus sp. nov., an antibiotic-producing actinobacterium isolated from bamboo (Sasa borealis) rhizosphere soil. . Int J Syst Evol Microbiol 62:, 856–859. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lee H. J. , Whang K. S. . ( 2014; ). Streptomyces graminilatus sp. nov., isolated from bamboo litter. . Int J Syst Evol Microbiol 64:, 528–532.[PubMed] [CrossRef]
    [Google Scholar]
  21. Manfio G. P. , Zakrzewska-Czerwinska J. , Atalan E. , Goodfellow M. . ( 1995; ). Towards minimal standards for the description of Streptomyces species. . Biotechnologiia 8:, 228–237.
    [Google Scholar]
  22. McCarthy A. J. , Williams S. T. . ( 1992; ). Actinomycetes as agents of biodegradation in the environment–a review. . Gene 115:, 189–192. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye G. , Schaa A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  25. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  27. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  28. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  29. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Waksman S. A. , Henrici A. T. . ( 1943; ). The nomenclature and classification of the actinomycetes. . J Bacteriol 46:, 337–341.[PubMed]
    [Google Scholar]
  31. Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. H. , Sneath P. H. A. , Sackin M. J. . ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  32. Williams S. T. , Goodfellow M. , Alderson G. . ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
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