1887

Abstract

A novel actinomycete, designated strain CA15-2, was isolated from a soil sample collected from the rhizosphere of tamarisk in the Lop Nor region, Xinjiang, China, and was characterized by using a polyphasic taxonomic approach. Optimal growth occurred at 37 °C and pH 7.5–8.0 and with 5 % (w/v) NaCl. Strain CA15-2 formed white to pale-yellow branched substrate mycelium without fragmentation and sparse aerial mycelium with wavelike curves. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the diagnostic diamino acid of the cell wall but no diagnostic sugars. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified glycolipid, one unidentified phospholipid and other unidentified lipids. MK-9(H), MK-10(H) and MK-10(H) were the predominant menaquinones. The major fatty acids were iso-C and C. The GC content of the genomic DNA was 69.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CA15-2 formed a distinct subclade in the family , with less than 95 % 16S rRNA gene sequence similarity to all known members of the family . On the basis of the evidence from our polyphasic study, a novel genus, gen. nov., is proposed, with the type species gen. nov., sp. nov. The type strain of is strain CA15-2 ( = DSM 45697 = CGMCC 4.7074).

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2015-01-01
2019-11-18
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