1887

Abstract

A Gram-stain-positive, non-spore-forming bacterium (strain RV113) was isolated from the marine sponge . 16S rRNA gene sequence analysis showed that strain RV113 belongs to the genus , and is related most closely to VF70612_S1 (96.9 % similarity) and more distantly related (<93 %) to all other species of the genus . The peptidoglycan diamino acid was lysine. Strain RV113 exhibited a quinone system with menaquinone MK-8 as the predominant compound. The polar lipid profile of strain RV113 consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso-Cω9. These chemotaxonomic traits are in agreement with those of other speciesof the genus . The results of physiological and biochemical tests allowed the clear phenotypic differentiation of strain RV113 from all recognized species. Strain RV113 is thus considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is RV113 ( = DSM 27440 = CECT 8425).

Funding
This study was supported by the:
  • , Deutsche Forschungsgemeinschaft , (Award SFB630-TP A5)

Erratum

This article contains a correction applying to the following content:
sp. nov. isolated from the marine sponge
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055152-0
2014-03-01
2020-09-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/705.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055152-0&mimeType=html&fmt=ahah

References

  1. Abdelmohsen U. R., Pimentel-Elardo S. M., Hanora A., Radwan M., Abou-El-Ela S. H., Ahmed S., Hentschel U. ( 2010 ). Isolation, phylogenetic analysis and anti-infective activity screening of marine sponge-associated actinomycetes. . Mar Drugs 8, 399412. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. ( 1996 ). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47, 3952. [CrossRef]
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 48014805. [CrossRef] [PubMed]
    [Google Scholar]
  4. Carreto L., Moore E., Nobre M. F., Wait R., Riley P. W., Sharp R. K., da Costa M. S. ( 1996 ). Rubrobacter xylanophilus sp. nov., a new thermophilic species isolated from a thermally polluted effluent. . Int J Syst Bacteriol 46, 460465. [CrossRef]
    [Google Scholar]
  5. Chen M. Y., Wu S. H., Lin G. H., Lu C. P., Lin Y. T., Chang W. C., Tsay S. S. ( 2004 ). Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs. . Int J Syst Evol Microbiol 54, 18491855. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Holmes A. J., Bowyer J., Holley M. P., O’Donoghue M., Montgomery M., Gillings M. R. ( 2000 ). Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils. . FEMS Microbiol Ecol 33, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  8. Imperi F., Caneva G., Cancellieri L., Ricci M. A., Sodo A., Visca P. ( 2007 ). The bacterial aetiology of rosy discoloration of ancient wall paintings. . Environ Microbiol 9, 28942902. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jurado V., Miller A. Z., Alias-Villegas C., Laiz L., Saiz-Jimenez C. ( 2012 ). Rubrobacter bracarensis sp. nov., a novel member of the genus Rubrobacter isolated from a biodeteriorated monument. . Syst Appl Microbiol 35, 306309. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227251. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  13. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [CrossRef] [PubMed]
    [Google Scholar]
  14. Schabereiter-Gurtner C., Piñar G., Vybiral D., Lubitz W., Rölleke S. ( 2001 ). Rubrobacter-related bacteria associated with rosy discolouration of masonry and lime wall paintings. . Arch Microbiol 176, 347354. [CrossRef] [PubMed]
    [Google Scholar]
  15. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [CrossRef] [PubMed]
    [Google Scholar]
  16. Suzuki K., Collins M. D., Iljima E., Komagata K. ( 1988 ). Chemotaxonomic characterization of a radiotolerant bacterium Arthrobacter radiotolerans: description of Rubrobacter radiotolerans gen. nov., comb. nov.. FEMS Microbiol Lett 52, 3340. [CrossRef]
    [Google Scholar]
  17. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  18. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  19. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 17431813.[PubMed]
    [Google Scholar]
  20. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yoshinaka T., Yano K., Yamaguchi H. ( 1973 ). Isolation of highly radioresistant bacterium Arthrobacter radiotolerans nov. sp.. Agric Biol Chem 37, 22692275. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055152-0
Loading
/content/journal/ijsem/10.1099/ijs.0.055152-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error