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A Gram-stain-positive, non-spore-forming bacterium (strain RV113T) was isolated from the marine sponge Aplysina aerophoba. 16S rRNA gene sequence analysis showed that strain RV113T belongs to the genus Rubrobacter , and is related most closely to Rubrobacter bracarensis VF70612_S1T (96.9 % similarity) and more distantly related (<93 %) to all other species of the genus Rubrobacter . The peptidoglycan diamino acid was lysine. Strain RV113T exhibited a quinone system with menaquinone MK-8 as the predominant compound. The polar lipid profile of strain RV113T consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso-C17 : 0ω9c. These chemotaxonomic traits are in agreement with those of other speciesof the genus Rubrobacter . The results of physiological and biochemical tests allowed the clear phenotypic differentiation of strain RV113T from all recognized Rubrobacter species. Strain RV113T is thus considered to represent a novel species, for which the name Rubrobacter aplysinae sp. nov. is proposed. The type strain is RV113T ( = DSM 27440T = CECT 8425T).
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