A taxonomic study was carried out on strain CY1T, which is a novel bacterium isolated from wastewater sludge of a melamine-producing factory in Sanming city, Fujian, China. Strain CY1T was shown to rapidly and completely degrade melamine to NH3 and CO2 under aerobic conditions. The isolate was Gram-stain-negative, short-rod-shaped and motile by one unipolar flagellum. Growth was observed at salinities from 0 to 7 % NaCl (optimum, 0.1 %), at temperatures from 15 to 50 °C (optimum, 40–45 °C) and at pH 7–9.5 (optimum pH 9.5). Quinone-8 was detected as the major respiratory quinone. 16S rRNA gene sequence comparisons showed that strain CY1T was affiliated to the family Comamonadaceae in the class Betaproteobacteria. It was most closely related to members of the genera Alicycliphilus (95.5 %), Diaphorobacter (94.6–95.1 %), Acidovorax (92.9–95.4 %), Delftia (93.0–93.6 %) and Comamonas (92.6–93.9 %). The average nucleotide identity (ANI) values between strain CY1T and those representing related genera ranged from 84.0 to 86.1 % using Mummer, and from 74.9 to 81.1 % using blast. The dominant fatty acids were C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0, C10 : 0 3-OH and C18 : 1ω7c and/or C18 : 1ω6c, and the major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminophospholipid. The G+C content of the chromosomal DNA was 69.5 mol%. On the basis of the phenotypic and phylogenetic data, strain CY1T represents a novel species of a new genus, for which the name Melaminivora alkalimesophila gen. nov., sp. nov. is proposed. The type strain of Melaminivora alkalimesophila is CY1T ( = CCTCC AB 2012024T = DSM 26006T).
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Funding
This study was supported by the:
Chinese Academy of Sciences
Technology Foundation for Selected Overseas Chinese Scholar of MOHRSS, China
China-EU Science & Technology Cooperation Program
(Award 2011DFA101222)
Science and Technology Planning Project of Xiamen, China
(Award 3502Z20120012 and 3502Z20102017)
CAS/SAFEA International Partnership Program for Creative Research Teams
(Award KZCX2-YW-T08)
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