@article{mbs:/content/journal/ijsem/10.1099/ijs.0.055103-0, author = "Wang, Han and Li, Jiangwei and Hu, Anyi and Qin, Dan and Xu, Heli and Yu, Chang-Ping", title = "Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory ", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_6", pages = "1938-1944", doi = "https://doi.org/10.1099/ijs.0.055103-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.055103-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A taxonomic study was carried out on strain CY1T, which is a novel bacterium isolated from wastewater sludge of a melamine-producing factory in Sanming city, Fujian, China. Strain CY1T was shown to rapidly and completely degrade melamine to NH3 and CO2 under aerobic conditions. The isolate was Gram-stain-negative, short-rod-shaped and motile by one unipolar flagellum. Growth was observed at salinities from 0 to 7 % NaCl (optimum, 0.1 %), at temperatures from 15 to 50 °C (optimum, 40–45 °C) and at pH 7–9.5 (optimum pH 9.5). Quinone-8 was detected as the major respiratory quinone. 16S rRNA gene sequence comparisons showed that strain CY1T was affiliated to the family Comamonadaceae in the class Betaproteobacteria . It was most closely related to members of the genera Alicycliphilus (95.5 %), Diaphorobacter (94.6–95.1 %), Acidovorax (92.9–95.4 %), Delftia (93.0–93.6 %) and Comamonas (92.6–93.9 %). The average nucleotide identity (ANI) values between strain CY1T and those representing related genera ranged from 84.0 to 86.1 % using Mummer, and from 74.9 to 81.1 % using blast. The dominant fatty acids were C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0, C10 : 0 3-OH and C18 : 1ω7c and/or C18 : 1ω6c, and the major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminophospholipid. The G+C content of the chromosomal DNA was 69.5 mol%. On the basis of the phenotypic and phylogenetic data, strain CY1T represents a novel species of a new genus, for which the name Melaminivora alkalimesophila gen. nov., sp. nov. is proposed. The type strain of Melaminivora alkalimesophila is CY1T ( = CCTCC AB 2012024T = DSM 26006T).", }