1887

Abstract

Urolithins are dibenzopyranone metabolites that exert anti-inflammatory activity and are produced by the gut microbiota from the dietary polyphenols ellagic acid (EA) and ellagitannins. However, the bacteria involved in this process remain unknown. We report here a novel bacterium, strain CEBAS 1/15P, capable of metabolizing EA to urolithins, that was isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods. The strain was related to 7-10-1-b, the type and only reported strain of the only species of the genus , with about 97 % 16S rRNA gene sequence similarity; they were both obligately anaerobic, non-spore-forming, Gram-stain-positive, short-rods/coccobacilli and metabolized only small numbers of carbon sources. -Fucose, -fructose, turanose, -galacturonic acid and α-ketobutyric acid were metabolized by strain CEBAS 1/15P, while was negative for metabolism of these compounds. The whole-cell fatty acids consisted predominantly of saturated fatty acids (70 %); strain CEBAS 1/15P differed significantly from in the major fatty acid, which was Cω9, while anteiso-C was the major component for . The presence of a number of different fatty acid peaks, especially C cyclo and Cω6, was also indicative of distinct species. Six glycolipids (GL1–6) were recognized, while, in , only four glycolipids were described. On the basis of these data, the novel species sp. nov. is described, with strain CEBAS 1/15P ( = DSM 27213 = CCUG 64261) as the type strain.

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2014-07-01
2020-01-29
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