1887

Abstract

Members of the haloarchaeal genera and (family ) are closely related to each other and show 96.6–98 % 16S rRNA gene sequence similarity. This is higher than the accepted threshold value (95 %) to separate two genera, and a taxonomic study using a polyphasic approach of all four members of the two genera was conducted to clarify their relationships. Polar lipid profiles indicated that RO1-4, BZ256 and RO1-6 are related more to each other than to CGMCC 1.6168. Phylogenetic analyses using the sequences of three different genes (16S rRNA gene, ′ and ) strongly supported the monophyly of these four species, showing that they formed a distinct clade, separate from the related genera , , , and . The results indicate that the four species should be assigned to the same genus, and it is proposed that and be transferred to the genus as comb. nov. (type strain, BZ256 = KCTC 4017 = JCM 14848) and comb. nov. (type strain, RO1-6 = CGMCC 1.8711 = JCM 16054).

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2013-10-01
2019-12-07
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