1887

Abstract

A Gram-staining-negative, rod-shaped and pink bacterium was isolated from the soil of a forest in the Taklamakan desert, Xinjiang, China. It was designated strain H9X. A 16S rRNA gene sequence homology search indicated that the isolate was most closely related to the family . The 16S rRNA gene of strain H9X displayed 94.2–96.3 % sequence identities to those of type strains of other species of the genus . It only possessed menaquinone-7. The major cellular fatty acids of the novel isolate were iso-C, Cω5 summed feature 3 (containing Cω6 and/or Cω7) and summed feature 4 (comprising anteiso-C B and/or iso-C I). The major polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, one unknown glycophospholipid and several unknown phospholipids. The DNA G+C content of this bacterium was 55.2 mol%. Based on the phenotypic and genotypic data presented, it can be concluded that this isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H9X ( = CCTCC AB 2013047 = KCTC 32396).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41072181 and 41272257)
  • Research Program for Biogeology and Environmental Geology (BGEG) Lab Staff (Award GBL11208)
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2014-03-01
2024-03-29
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References

  1. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868. [PubMed]
    [Google Scholar]
  2. Dastager S. G., Raziuddin Q. S., Deepa C. K., Li W. J., Pandey A. ( 2010 ). Pontibacter niistensis sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60, 28672870. [View Article] [PubMed]
    [Google Scholar]
  3. Dwivedi V., Niharika N., Lal R. ( 2013 ). Pontibacter lucknowensis sp. nov., isolated from a hexachlorocyclohexane dump site. . Int J Syst Evol Microbiol 63, 309313. [View Article] [PubMed]
    [Google Scholar]
  4. Fautz E., Reichenbach H. ( 1980 ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8, 8791. [View Article]
    [Google Scholar]
  5. Joshi M. N., Sharma A. C., Pandya R. V., Patel R. P., Saiyed Z. M., Saxena A. K., Bagatharia S. B. ( 2012 ). Draft genome sequence of Pontibacter sp. nov. BAB1700, a halotolerant, industrially important bacterium. . J Bacteriol 194, 63296330. [View Article] [PubMed]
    [Google Scholar]
  6. Kang J. Y., Joung Y., Chun J., Kim H., Joh K., Jahng K. Y. ( 2013 ). Pontibacter saemangeumensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 63, 565569. [View Article] [PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  8. Komagata K., Suzuki K. ( 1987 ). Lipidandcellwallanalysisin bacterialsystematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  9. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115176. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+C content of desoxyribonucleic acids by high performance liquid chromatography. . Int Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  12. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. & other authors ( 2005 ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55, 25832588. [View Article] [PubMed]
    [Google Scholar]
  13. Singh A. K., Garg N., Sangwan N., Negi V., Kumar R., Vikram S., Lal R. ( 2013 ). Pontibacter ramchanderi sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment. . Int J Syst Evol Microbiol 63, 28292834. [View Article] [PubMed]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  15. Suresh K., Mayilraj S., Chakrabarti T. ( 2006 ). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family “Flexibacteraceae”. . Int J Syst Evol Microbiol 56, 17031707. [View Article] [PubMed]
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  17. Wang Y., Zhang K., Cai F., Zhang L., Tang Y., Dai J., Fang C. ( 2010 ). Pontibacter xinjiangensis sp. nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov.. Int J Syst Evol Microbiol 60, 99103. [View Article] [PubMed]
    [Google Scholar]
  18. Xu M., Wang Y., Dai J., Jiang F., Rahman E., Peng F., Fang C. ( 2012 ). Pontibacter populi sp. nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest. . Int J Syst Evol Microbiol 62, 665670. [View Article] [PubMed]
    [Google Scholar]
  19. Zhang L., Zhang Q., Luo X., Tang Y., Dai J., Li Y., Wang Y., Chen G., Fang C. ( 2008 ). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 58, 12101214. [View Article] [PubMed]
    [Google Scholar]
  20. Zhou Y., Wang X., Liu H., Zhang K. Y., Zhang Y. Q., Lai R., Li W. J. ( 2007 ). Pontibacter akesuensis sp. nov., isolated from a desert soil in China. . Int J Syst Evol Microbiol 57, 321325. [View Article] [PubMed]
    [Google Scholar]
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