1887

Abstract

The taxonomic position of Eg1 ( = JCM 15918) based on comparative 16S rRNA gene and sequence analyses was found to be controversial, as the strain showed high similarity to the type strain of , CCUG 18363. Therefore, the relationship between the two species was investigated by a taxonomic study that included, in addition to re-evaluation of the 16S rRNA gene sequence, determination of DNA–DNA binding and multilocus sequence analysis (MLSA) of housekeeping genes encoding the DNA-directed RNA polymerase B subunit (), putative xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (), elongation factor EF-G (), 50S ribosomal protein L2 () and DNA gyrase B subunit (). Comparative 16S rRNA gene sequence analysis showed relatively high similarity (98.9 %) between KCTC 5756 and ATCC 15703. MLSA revealed close relatedness between KCTC 5756 and CCUG 18363, with 99.3–100 % similarity between the , , , and gene sequences. In addition, relatively high gene sequence similarity of 97.7 % was found between the strains. Similar phenotypes and a high DNA–DNA binding value (78.9 %) confirmed that and are synonymous. Based on these results, it is proposed that the species Kim 2010 should be reclassified as a later heterotypic synonym of Reuter 1963 (Approved Lists 1980).

Funding
This study was supported by the:
  • , Czech Science Foundation , (Award GA CR 304/11/1252)
  • , Operation Programme Education for Competitiveness project CEB , (Award CZ.1.07/2.3.00/20.0183)
  • , Institutional Research Project of the Institute of Animal Physiology and Genetics, Acad. Sci. CR , (Award RVO 67985904)
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2013-11-01
2020-07-15
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