1887

Abstract

Two Gram-reaction-negative, aerobic, nitrogen-fixing, rod-shaped bacteria, designated strains E20 and E50, were isolated from the rhizosphere of salt meadow plants and , respectively, near Münzenberg, Germany. Based on the 16S rRNA gene sequence analysis both strains E20 and E50 are affiliated with the genus , sharing the highest similarity with LMG 18551 (96.4 %) and (97.1 %), respectively. Strains E20 and E50 were oxidase and catalase-positive, grew at a temperature range between 16 and 37 °C and in the presence of 0–5 % NaCl (w/v). The DNA G+C contents were 52.1 mol% (E20) and 51.6 mol% (E50). Major fatty acids of strains E20 and E50 were summed feature 3 (Cω7 and/or iso-C 2-OH), C, Cω7, C, C and C 3-OH. The DNA–DNA relatedness of the strains to LMG 18551 was 39 % for strain E20 and 58 % for strain E50. The nitrogen fixation capability of strains E20 and E50 was confirmed by the acetylene reduction assay. On the basis of our polyphasic taxonomic study, strains E20 and E50 represent a novel species of the genus , for which the name is proposed. The type strain of is E50 ( = LMG 27267 = KACC 17069). An emended description of the genus is proposed based on the capability of fixing nitrogen and growth in presence of up to 5 % NaCl (w/v).

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2014-02-01
2021-07-25
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