1887

Abstract

A novel aerobic marine bacterium, strain AN44, was isolated from the coral sampled from the Andaman Sea, India. Cells were Gram-negative, motile and rod-shaped. Oxidase and catalase tests were positive. Heterotrophic growth was observed at pH 5.5–10 and at 16–42 °C, with optimum growth at pH 7–8 and 28 °C. Strain AN44 grew in the presence of 0.5–11 % (w/v) NaCl; the optimal NaCl concentration for growth was 3–5 %. The DNA G+C content was 47.8 mol%. Predominant cellular fatty acids of strain AN44 were Cω7, Cω7/Cω6, C, C 3-OH, C, C, C and C. The sole isoprenoid ubiquinone was Q-8. The polar lipids were an unidentified phospholipid, an unidentified aminophospholipid and two unidentified glycolipids. 16S rRNA gene sequence comparisons revealed that strain AN44 clustered within the radiation of the genus and showed similarity of 97.9 % with UST010306-043, 97.8 % with 11SM4, 97.1 % with R-40503 and 97.0 % with 8. However, DNA–DNA relatedness between strain AN44 and closely related type strains was well below 70 %. On the basis of the data from the present polyphasic taxonomic study, strain AN44 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AN44 ( = JCM 18476 = LMG 27065).

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2014-02-01
2019-10-23
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  2. Bandyopadhyay S., Schumann P., Das S. K.. ( 2013;). Pannonibacter indica sp. nov., a highly arsenate-tolerant bacterium isolated from a hot spring in India. . Arch Microbiol 195:, 1–8. [CrossRef][PubMed]
    [Google Scholar]
  3. Baumann L., Baumann P., Mandel M., Allen R. D.. ( 1972;). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110:, 402–429.[PubMed]
    [Google Scholar]
  4. Bligh E. G., Dyer W. J.. ( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37:, 911–917. [CrossRef][PubMed]
    [Google Scholar]
  5. Chang H. W., Roh S. W., Kim K. H., Nam Y. D., Yoon J. H., Oh H. M., Bae J. W.. ( 2008;). Marinomonas basaltis sp. nov., a marine bacterium isolated from black sand. . Int J Syst Evol Microbiol 58:, 2743–2747. [CrossRef][PubMed]
    [Google Scholar]
  6. Chimetto L. A., Brocchi M., Gondo M., Thompson C. C., Gomez-Gil B., Thompson F. L.. ( 2009;). Genomic diversity of vibrios associated with the Brazilian coral Mussismilia hispida and its sympatric zoanthids (Palythoa caribaeorum, Palythoa variabilis and Zoanthus solanderi). . J Appl Microbiol 106:, 1818–1826. [CrossRef][PubMed]
    [Google Scholar]
  7. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L.. ( 2011;). Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis. . Int J Syst Evol Microbiol 61:, 1170–1175. [CrossRef][PubMed]
    [Google Scholar]
  8. Dinsdale E. A., Pantos O., Smriga S., Edwards R. A., Angly F., Wegley L., Hatay M., Hall D., Brown E.. & other authors ( 2008;). Microbial ecology of four coral atolls in the Northern Line Islands. . PLoS ONE 3:, e1584. [CrossRef][PubMed]
    [Google Scholar]
  9. Espinosa E., Marco-Noales E., Gómez D., Lucas-Elío P., Ordax M., Garcías-Bonet N., Duarte C. M., Sanchez-Amat A.. ( 2010;). Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.. Int J Syst Evol Microbiol 60:, 93–98. [CrossRef][PubMed]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  11. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  12. Guppy R., Bythell J. C.. ( 2006;). Environmental effects on bacterial diversity in the surface mucus layer of the reef coral Montastraea faveolata. . Mar Ecol Prog Ser 328:, 133–142. [CrossRef]
    [Google Scholar]
  13. Gupta P., Chaturvedi P., Pradhan S., Delille D., Shivaji S.. ( 2006;). Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water off the subantarctic Kerguelen islands. . Int J Syst Evol Microbiol 56:, 361–364. [CrossRef][PubMed]
    [Google Scholar]
  14. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A.. ( 2005;). Marinomonas pontica sp. nov., isolated from the Black Sea. . Int J Syst Evol Microbiol 55:, 275–279. [CrossRef][PubMed]
    [Google Scholar]
  15. Jung Y. T., Oh T. K., Yoon J. H.. ( 2012;). Marinomonas hwangdonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62:, 2062–2067. [CrossRef][PubMed]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  18. Koren O., Rosenberg E.. ( 2006;). Bacteria associated with mucus and tissues of the coral Oculina patagonica in summer and winter. . Appl Environ Microbiol 72:, 5254–5259. [CrossRef][PubMed]
    [Google Scholar]
  19. Kumari P., Bandyopadhyay S., Das S. K.. ( 2013;). Microbacterium oryzae sp. nov., an actinobacterium isolated from rice field soil. . Int J Syst Evol Microbiol 63:, 2442–2449. [CrossRef][PubMed]
    [Google Scholar]
  20. Laemmli U. K.. ( 1970;). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. . Nature 227:, 680–685. [CrossRef][PubMed]
    [Google Scholar]
  21. Lau K. W., Ren J., Wai N. L., Lau S. C., Qian P. Y., Wong P. K., Wu M.. ( 2006;). Marinomonas ostreistagni sp. nov., isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China. . Int J Syst Evol Microbiol 56:, 2271–2275. [CrossRef][PubMed]
    [Google Scholar]
  22. Lewin R. A., Lounsbery D. M.. ( 1969;). Isolation, cultivation and characterization of flexibacteria. . J Gen Microbiol 58:, 145–170. [CrossRef][PubMed]
    [Google Scholar]
  23. Littman R. A., Willis B. L., Pfeffer C., Bourne D. G.. ( 2009;). Diversities of coral-associated bacteria differ with location, but not species, for three acroporid corals on the Great Barrier Reef. . FEMS Microbiol Ecol 68:, 152–163. [CrossRef][PubMed]
    [Google Scholar]
  24. Lucas-Elío P., Marco-Noales E., Espinosa E., Ordax M., López M. M., Garcías-Bonet N., Marbà N., Duarte C. M., Sanchez-Amat A.. ( 2011;). Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica. . Int J Syst Evol Microbiol 61:, 2191–2196. [CrossRef][PubMed]
    [Google Scholar]
  25. Macián M. C., Arahal D. R., Garay E., Pujalte M. J.. ( 2005;). Marinomonas aquamarina sp. nov., isolated from oysters and seawater. . Syst Appl Microbiol 28:, 145–150. [CrossRef][PubMed]
    [Google Scholar]
  26. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  27. Panday D., Das S. K.. ( 2010;). Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment. . Int J Syst Evol Microbiol 60:, 861–865. [CrossRef][PubMed]
    [Google Scholar]
  28. Prabagaran S. R., Suresh K., Manorama R., Delille D., Shivaji S.. ( 2005;). Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. . Int J Syst Evol Microbiol 55:, 309–313. [CrossRef][PubMed]
    [Google Scholar]
  29. Rohwer F., Breitbart M., Jara J., Azam F., Knowlton N.. ( 2001;). Diversity of bacteria associated with the Caribbean coral Montastraea franksi. . Coral Reefs 20:, 85–91. [CrossRef]
    [Google Scholar]
  30. Rohwer F., Seguritan V., Azam F., Knowlton N.. ( 2002;). Diversity and distribution of coral-associated bacteria. . Mar Ecol Prog Ser 243:, 1–10. [CrossRef]
    [Google Scholar]
  31. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T.. ( 2003;). Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. . Int J Syst Evol Microbiol 53:, 829–832. [CrossRef][PubMed]
    [Google Scholar]
  32. Romanenko L. A., Uchino M., Frolova G. M., Mikhailov V. V.. ( 2007;). Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. . Int J Syst Evol Microbiol 57:, 457–462. [CrossRef][PubMed]
    [Google Scholar]
  33. Romanenko L. A., Tanaka N., Frolova G. M.. ( 2009;). Marinomonas arenicola sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 59:, 2834–2838. [CrossRef][PubMed]
    [Google Scholar]
  34. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  35. Sambrook J., Russell D. W.. ( 2001;). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  36. Sanchez-Amat A., Solano F.. ( 2005;). Genus III. Marinomonas Van Landschoot and De Ley 1984, 91VP (effective publication: Van Landschoot and De Ley 1983, 3071). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 284–289. Edited by Brenner D. J., Krieg N. R., Staley J. T... New York:: Springer;.
    [Google Scholar]
  37. Shieh W. Y., Lin Y.-T., Jean W. D.. ( 2004;). Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. . Int J Syst Evol Microbiol 54:, 2307–2312. [CrossRef][PubMed]
    [Google Scholar]
  38. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  39. Solano F., Sanchez-Amat A.. ( 1999;). Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov.. Int J Syst Bacteriol 49:, 1241–1246. [CrossRef][PubMed]
    [Google Scholar]
  40. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  41. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  42. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  43. van Landschoot A., De Ley J.. ( 1983;). Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other gram-negative bacteria. . J Gen Microbiol 129:, 3057–3074.
    [Google Scholar]
  44. Vauterin L., Vauterin P.. ( 1992;). Computer aided objective comparison of electrophoretic patterns for grouping and identification of microorganisms. . Eur Microbiol 1:, 37–41.
    [Google Scholar]
  45. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  46. Yoon J. H., Kang S. J., Oh T. K.. ( 2005;). Marinomonas dokdonensis sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 55:, 2303–2307. [CrossRef][PubMed]
    [Google Scholar]
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