1887

Abstract

A thallium-tolerant, aerobic bacterium, designated strain SK200a-9, isolated from a garden soil sample was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that strain SK200a-9 was affiliated with an uncultivated lineage within the and the nearest cultivated neighbours were bacteria in genera in the family (93.3–94.4 % 16S rRNA gene sequence similarity) and the family (92.3–93.1 %) in the order . Cells of strain SK200a-9 were Gram-stain-negative, non-motile, non-spore-forming, poly-β-hydroxybutyrate-accumulating rods. The strain was a chemo-organotrophic bacterium, which was incapable of growth on C1 substrates. Catalase and oxidase were positive. Atmospheric nitrogen fixation and nitrate reduction were negative. The strain contained ubiquinone Q-10 and cellular fatty acids Cω7, C, Cω7 and C as predominant components. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 64.8 mol%. On the basis of the information described above, strain SK200a-9 is considered to represent a novel species of a new genus in the order , for which the name gen. nov., sp. nov. is proposed. The type strain of is SK200a-9 ( = NBRC 107718 = CGMCC 1.12214).

Funding
This study was supported by the:
  • , Yuuji Ushiba
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2014-03-01
2020-09-21
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