1887

Abstract

A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterium, designated strain GSR3061, was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GSR3061 indicated that the isolate belonged to the phylum and exhibited similarity levels of 94.7 % to NRRL B-51285, 92.4 % to KACC 14257 and 91.9 % to KACC 15371. Growth was observed at 15–40 °C and pH 6.5–9.5. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-C I and/or anteiso-C B), iso-C, Cω6 and iso-C H. Flexirubin-type pigments were absent. The DNA G+C content of strain GSR3061 was 44.9 mol% and the major quinone was MK-7. The polar lipid profile consisted mainly of phosphatidylethanolamine; three unidentified lipids, two unknown aminolipids, two unknown phospholipids, an unknown aminophospholipid and an unknown glycolipid were also present. On the basis of the evidence presented, it is concluded that strain GSR3061 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is GSR3061 ( = KACC 16450 = JCM 17917).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.054569-0
2013-12-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4663.html?itemId=/content/journal/ijsem/10.1099/ijs.0.054569-0&mimeType=html&fmt=ahah

References

  1. Abaydulla G., Luo X., Shi J., Peng F., Liu M., Wang Y., Dai J., Fang C.. ( 2012;). Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil. . Antonie van Leeuwenhoek 101:, 725–731. [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Buczolits S., Denner E. B., Kämpfer P., Busse H.-J.. ( 2006;). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. . Int J Syst Evol Microbiol 56:, 2071–2078. [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M.. ( 1985;). Isoprenoid quinone analyses in bacterial classification and identification. . Soc Appl Bacteriol Tech Ser, 267–287.
    [Google Scholar]
  6. Connon S. A., Giovannoni S. J.. ( 2002;). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. . Appl Environ Microbiol 68:, 3878–3885. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Filippini M., Kaech A., Ziegler U., Bagheri H. C.. ( 2011a;). Fibrisoma limi gen. nov., sp. nov., a filamentous bacterium isolated from tidal flats. . Int J Syst Evol Microbiol 61:, 1418–1424. [CrossRef][PubMed]
    [Google Scholar]
  10. Filippini M., Svercel M., Laczko E., Kaech A., Ziegler U., Bagheri H. C.. ( 2011b;). Fibrella aestuarina gen. nov., sp. nov., a filamentous bacterium of the family Cytophagaceae isolated from a tidal flat, and emended description of the genus Rudanella Weon et al. 2008. . Int J Syst Evol Microbiol 61:, 184–189. [CrossRef][PubMed]
    [Google Scholar]
  11. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Biol 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Jiang F., Xiao M., Chen L., Kan W., Peng F., Dai J., Chang X., Li W., Fang C.. ( 2013;). Huanghella arctica gen. nov., sp. nov., a bacterium of the family Cytophagaceae isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 63:, 696–702. [CrossRef][PubMed]
    [Google Scholar]
  13. Kämpfer P., Busse H.-J., Longaric I., Rosselló-Móra R., Galatis H., Lodders N.. ( 2012;). Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis. . Int J Syst Evol Microbiol 62:, 2247–2251. [CrossRef][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  16. Minnikin D., O’Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Nakagawa Y.. ( 2011;). Family I. Cytophagaceae Stanier 1940, 630AL. . In Bergey’s Manual of Systemic Bacteriology 4. pp. 371–423. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Scherer P., Kneifel H.. ( 1983;). Distribution of polyamines in methanogenic bacteria. . J Bacteriol 154:, 1315–1322.[PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  23. Tittsler R. P., Sandholzer L. A.. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  24. Zhang L., Zhang Q., Luo X., Tang Y., Dai J., Li Y., Wang Y., Chen G., Fang C.. ( 2008;). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 58:, 1210–1214. [CrossRef][PubMed]
    [Google Scholar]
  25. Zhang J. Y., Liu X. Y., Liu S. J.. ( 2009;). Adhaeribacter terreus sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 59:, 1595–1598. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.054569-0
Loading
/content/journal/ijsem/10.1099/ijs.0.054569-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error