1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_12

gen. nov., sp. nov., an anaerobic, alkalithermophilic bacterium from soda lake sediment Free

Abstract

An obligately alkaliphilic, anaerobic, thermo- and halotolerant, spore-forming bacterium was isolated from sediments of soda lake Magadi (Kenya) and designated strain Z-1001. Cells of strain Z-1001 were straight, Gram-positive rods, slowly motile. Strain Z-1001 was found to be an obligate anaerobe. It grew within a pH range from 7.5 to 10.7 with an optimum at 9.25–9.5 (at 40 °C), a temperature range from 20 to 57 °C with an optimum at 45–50 °C, and a NaCl concentration range from 0 to 1.55 M with an optimum at 1.2–1.4 M. Peptides, such as meat and yeast extracts, peptone and tryptone, were fermented by Z-1001. Carbohydrates did not support growth. With yeast extract as an electron donor, strain Z-1001 reduced , , , Fe(III) citrate and anthraquinone-2,6-disulfonate (AQDS) as electron acceptors. The isolate was able to grow oligotrophically with a very small amount of yeast extract: 0.03 g l. The main fatty acids were C, C, C and C. The DNA G+C content of the isolate was 35.6 mol%. 16S rRNA gene sequence analysis showed that strain Z-1001 is a member of family , clustering with the type strain of (95.8–96.0 % sequence similarity). On the basis of physiological and phylogenetic data it is proposed that strain Z-1001 ( = DSM 24923 = VKM B-2666) represents a novel genus and species, gen. nov., sp. nov.

  • Published Online:
Funding
This study was supported by the:
  • Russian Academy of Sciences (Award 02.552.11.7073 and 02.740.11.0023)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.054536-0
2013-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4456.html?itemId=/content/journal/ijsem/10.1099/ijs.0.054536-0&mimeType=html&fmt=ahah

References

  1. Boulygina E. S., Kuznetsov B. B., Marusina A. I., Tourova T. P., Kravchenko I. K., Bykova S. A., Kolganova T. V., Galchenko V. F. ( 2002 ). A study of nucleotide sequences of nifH genes of some methanotrophic bacteria. . Microbiology (English translation of Mikrobiologiya) 71, 425–432. [View Article]
    [Google Scholar]
  2. Christie W. W. ( 2011 ). Thin-layer chromatography of lipids. . http://lipidlibrary.aocs.org/topics/tlc/index.htm
    [Google Scholar]
  3. Duckworth A. W., Grant W. D., Jones B. E., van Steenbergen R. ( 1996 ). Phylogenetic diversity of soda lake alkaliphiles. . FEMS Microbiol Ecol 19, 181–191. [View Article]
    [Google Scholar]
  4. Garnova E. S., Zhilina T. N., Tourova T. P., Lysenko A. M. ( 2003 ). Anoxynatronum sibiricum gen.nov., sp.nov. alkaliphilic saccharolytic anaerobe from cellulolytic community of Nizhnee Beloe (Transbaikal region). . Extremophiles 7, 213–220.[PubMed]
    [Google Scholar]
  5. Grant W. D., Mwatha W. E., Jones B. E. ( 1990 ). Alkaliphiles: ecology, diversity and applications. . FEMS Microbiol Rev 75, 255–269. [View Article]
    [Google Scholar]
  6. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 95–98.
     [Google Scholar]
  7. Jones B. E., Grant W. D., Duckworth A. W., Owenson G. G. ( 1998 ). Microbial diversity of soda lakes. . Extremophiles 2, 191–200. [View Article] [PubMed]
    [Google Scholar]
  8. Kevbrin V. V., Zavarzin G. A. ( 1992 ). The effect of sulfur compounds on growth of halophilic the homoacetic bacterium Acetohalobium arabaticum . . Microbiology (English translation of Mikrobiologiya) 61, 563–567.
     [Google Scholar]
  9. Lane D. J. ( 1991 ). 16S/23S sequencing. . In Nucleic Acid Techniques In Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  10. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  11. Mesbah N. M., Wiegel J. ( 2009 ). Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt. . Int J Syst Evol Microbiol 59, 2042–2048. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah N. M., Hedrick D. B., Peacock A. D., Rohde M., Wiegel J. ( 2007 ). Natranaerobius thermophilus gen. nov., sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt, and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov.. Int J Syst Evol Microbiol 57, 2507–2512. [View Article] [PubMed]
    [Google Scholar]
  13. Owen R. J., Hill L. R., Lapage S. P. ( 1969 ). Determination of DNA base compositions from melting profiles in dilute buffers. . Biopolymers 7, 503–516. [View Article] [PubMed]
    [Google Scholar]
  14. Rees H. C., Grant W. D., Jones B. E., Heaphy S. ( 2004 ). Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods. . Extremophiles 8, 63–71. [View Article] [PubMed]
    [Google Scholar]
  15. Stookey L. L. ( 1970 ). Ferrozine – a new spectrophotometric reagent for iron. . Anal Chem 42, 779–781. [View Article]
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 4673–4680. [View Article] [PubMed]
    [Google Scholar]
  18. Tindall B. J. ( 1988 ). Prokaryotic life in the alkaline, saline, athalassic environment. . In Halophilic Bacteria, vol. 1, pp. 31–67. Edited by Rodriguez-Valera F. . Boca Raton, FL:: CRC Press;.
     [Google Scholar]
  19. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  20. Trüper H. G., Schlegel H. G. ( 1964 ). Sulfur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells of Chromatium okenii . . Antonie van Leeuwenhoek 30, 225–238. [View Article] [PubMed]
    [Google Scholar]
  21. Vaskovsky V. E., Kostetsky E. Y. ( 1968 ). Modified spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res 9, 396.[PubMed]
    [Google Scholar]
  22. Zavarzin G. A. ( 1993 ). Epicontinental soda lakes are probable relict biotopes of terrestrial biota formation. . Microbiology 62, 473–479. (English translation of Mikrobiologiya).
     [Google Scholar]
  23. Zavarzina D. G., Tourova T. P., Kuznetsov B. B., Bonch-Osmolovskaya E. A., Slobodkin A. I. ( 2002 ). Thermovenabulum ferriorganovorum gen. nov., sp. nov., a novel thermophilic, anaerobic, endospore-forming bacterium. . Int J Syst Evol Microbiol 52, 1737–1743. [View Article] [PubMed]
    [Google Scholar]
  24. Zavarzina D. G., Kolganova T. V., Bulygina E. S., Kostrikina N. A., Turova T. P., Zavarzin G. A. ( 2006 ). [Geoalkalibacter ferrihydriticus gen. nov., sp. nov., the first alkaliphilic representative of the family Geobacteraceae, isolated from a soda lake]. . Mikrobiologiia 75, 775–785 (In Russian).[PubMed]
    [Google Scholar]
  25. Zavarzina D. G., Tourova T. P., Kolganova T. V., Boulygina E. S., Zhilina T. N. ( 2009 ). Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly anaerobic diazotrophic bacillus isolated from soda lake and transfer of Bacillus arseniciselenatis, Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the new combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov.. Microbiology (English translation of Mikrobiologiya) 78, 723–731. [View Article]
    [Google Scholar]
  26. Zhilina T. N., Zavarzin G. A. ( 1994 ). Alkaliphilic anaerobic community at pH 10. . Curr Microbiol 29, 109–112. [View Article]
    [Google Scholar]
  27. Zhilina T. N., Zavarzina D. G., Panteleeva A. N., Osipov G. A., Kostrikina N. A., Tourova T. P., Zavarzin G. A. ( 2012 ). Fuchsiella alkaliacetigena gen. nov., sp. nov., an alkaliphilic, lithoautotrophic homoacetogen from a soda lake. . Int J Syst Evol Microbiol 62, 1666–1673. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.054536-0
Loading
Natranaerobaculum magadiense gen. nov., sp. nov., an anaerobic, alkalithermophilic bacterium from soda lake sediment
Int J Syst Evol Microbiol 63, 4456 (2013); https://doi.org/10.1099/ijs.0.054536-0
/content/journal/ijsem/10.1099/ijs.0.054536-0
/content/journal/ijsem/10.1099/ijs.0.054536-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error