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Abstract

As part of an undergraduate microbiology course, a yellow–orange-pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain was isolated from a glass tank housing several red-spotted newts (). The sequence of the 16S rRNA gene of this strain, designated KM, was 97.4–98.0 % similar to those of the type strains of , and , while the similarity levels for protein-coding genes were less than 94.7 % for , less than 92.1 % for and less than 87.1 % for . These values are lower than for many other established distinct species. Polyphasic characterization and comparison to these relatives revealed that strain KM was similar to other strains in that it contained MK-6 as its major respiratory quinone and phosphatidylethanolamine as the most abundant polar lipid, produced flexirubin-type pigments, oxidase and catalase and primarily contained the fatty acids iso-C, iso-Cω9, iso-C 3-OH and summed feature 3 (comprising Cω6 and/or Cω7). Based on the results of this study, strain KM represents a novel species, for which the name sp. nov. is proposed. The type strain is KM ( = ATCC BAA-2160 = NRRL B-59516 = KCTC 23297).

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2013-12-01
2019-10-17
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