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Abstract

Two Gram-staining-negative, strictly aerobic, non-endospore-forming, non-motile, rod-shaped bacteria, designated strains YIT 12745 and YIT 12746, were isolated from sludge from a wastewater treatment plant. 16S rRNA gene sequence analyses indicated that these strains belonged to the genus . In these analyses, strains YIT 12745 and YIT 12746 were most closely related to the type strains of and , with 16S rRNA gene sequence similarity values of 94.9 % and 96.2 %, respectively. For both novel strains, menaquinone (MK-6) was the only respiratory quinone. The major fatty acids of strain YIT 12745 were iso-C G (14.4 %), iso-C (13.2 %), C (12.9 %), iso-C (12.9 %) and iso-C 3-OH (11.5 %). Those of strain YIT 12746 were iso-C (21.5 %), iso-C (13.3 %), C (12.0 %) and iso-C G (11.9 %). The genomic DNA G+C contents of strains YIT 12745 and YIT 12746 were 48.7 and 30.9 mol%, respectively. From their differential phenotypic and phylogenetic characteristics, these strains are considered to represent two novel species of the genus , for which the names sp. nov. (type strain YIT 12745 = JCM 19141 = DSM 27077) and sp. nov. (type strain YIT 12746 = JCM 19142 = DSM 27078) are proposed. Emended descriptions of and are also proposed.

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2014-05-01
2024-12-06
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References

  1. Aslam Z., Im W.-T., Kim M. K., Lee S.-T. ( 2005 ). Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. . Int J Syst Evol Microbiol 55, 747751. [View Article] [PubMed]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  3. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) ( 1923 ). Genus II. Flavobacterium gen. nov.. In Bergey’s Manual of Determinative Bacteriology, pp. 97117. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  4. Bernardet J.-F., Bowman J. P. ( 2011 ). Genus I. Flavobacterium Bergey et al. 1923. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 112154. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  5. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996 ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46, 128148. [View Article]
    [Google Scholar]
  6. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  7. Ezaki T., Saidi S. M., Liu S. L., Hashimoto Y., Yamamoto H., Yabuuchi E. ( 1990 ). Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. . FEMS Microbiol Lett 67, 127130. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Biol 20, 406416. [View Article]
    [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [View Article]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  13. Kirchman D. L. ( 2002 ). The ecology of Cytophaga-Flavobacteria in aquatic environments. . FEMS Microbiol Ecol 39, 91100.[PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  15. Liu Y., Jin J.-H., Zhou Y.-G., Liu H.-C., Liu Z.-P. ( 2010 ). Flavobacterium caeni sp. nov., isolated from a sequencing batch reactor for the treatment of malachite green effluents. . Int J Syst Evol Microbiol 60, 417421. [View Article] [PubMed]
    [Google Scholar]
  16. Ludwig W., Euzéby J., Whitman W. B. ( 2011 ). Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 2124. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  18. Montero-Calasanz M. C., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M. ( 2013 ). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. . Int J Syst Evol Microbiol 63, 43864395. [View Article] [PubMed]
    [Google Scholar]
  19. Nokhal T. H., Schlegel H. G. ( 1983 ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33, 2637. [View Article]
    [Google Scholar]
  20. Park M., Lu S., Ryu S. H., Chung B. S., Park W., Kim C.-J., Jeon C. O. ( 2006 ). Flavobacterium croceum sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 56, 24432447. [View Article] [PubMed]
    [Google Scholar]
  21. Park M., Ryu S. H., Vu T.-H. T., Ro H.-S., Yun P.-Y., Jeon C. O. ( 2007 ). Flavobacterium defluvii sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 57, 233237. [View Article] [PubMed]
    [Google Scholar]
  22. Ryu S. H., Park M., Jeon Y., Lee J. R., Park W., Jeon C. O. ( 2007 ). Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea. . Int J Syst Evol Microbiol 57, 20262030. [View Article] [PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  24. Sakon H., Nagai F., Morotomi M., Tanaka R. ( 2008 ). Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 58, 970975. [View Article] [PubMed]
    [Google Scholar]
  25. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. . Newark, DE:: MIDI;.
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  27. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Bacteriology, , 3rd edn., pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60, 249266. [View Article] [PubMed]
    [Google Scholar]
  29. Yoon J. H., Kang S. J., Lee J. S., Oh T. K. ( 2007 ). Flavobacterium terrigena sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57, 947950. [View Article] [PubMed]
    [Google Scholar]
  30. Zhang J., Jiang R.-B., Zhang X.-X., Hang B.-J., He J., Li S.-P. ( 2010 ). Flavobacterium haoranii sp. nov., a cypermethrin-degrading bacterium isolated from a wastewater treatment system. . Int J Syst Evol Microbiol 60, 28822886. [View Article] [PubMed]
    [Google Scholar]
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