1887

Abstract

Three Gram-stain-positive bacterial strains, 11050, 7-19 and 11102, were isolated from traditional pickle and sourdough in Heilongjiang Province, China. These bacteria were characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA–DNA hybridization and an analysis of phenotypic features. Strain 11050 belonged to the species group and shared 98.0–98.4 % 16S rRNA gene sequence similarities and 84.7–88.9 % gene sequence similarities with type strains of , , , , and and had 75.9–80.7 % gene sequence similarities and 90.7–92.5 % gene sequence similarities with LMG 6907, LMG 9205, LMG 10755, LMG 16673, LMG 24284 and 3.1.1, respectively. Strain 7-19 was phylogenetically related to , and , having 94.1–96.7 % 16S rRNA gene sequence similarities, 71.5–82.3 % gene sequence similarities and 71.2–83.4 % gene sequence similarities with type strains of , and , respectively. Strain 11102 was phylogenetically related to , , and . Strain 11102 had 99.2 % 16S rRNA gene sequence similarity, 81.3 % gene sequence similarity and 96.1 % gene sequence similarity with LMG 22743, respectively. Strain 11102 shared 96.0–96.8 % 16S rRNA gene sequence similarities, 73.3–81.0 % gene sequence similarities and 74.6–76.9 % gene sequence similarities with type strains of , and , respectively. Based upon the data from polyphasic characterization obtained in the present study, three novel species, sp. nov., sp. nov. and sp. nov., are proposed and the type strains are 11050 ( = LMG 27194 = CCUG 62991), 7-19 ( = LMG 27191 = NCIMB 14832 = CCUG 62990) and 11102 ( = LMG 27192 = NCIMB 14833), respectively.

Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2012AA022108)
  • Program for Changjiang Scholars and Innovative Research Team in University (Award IRT0959)
  • National Natural Science Foundation of China (Award 31101342)
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2013-12-01
2021-10-23
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