1887

Abstract

Three strictly anaerobic, methanogenic strains JH1, JH4 and JH8 were isolated from rumen of the Korean native cattle (HanWoo; ) in South Korea. The colonies were circular, opaque, and slightly yellowish. Phylogenetic analyses of 16S rRNA gene and (encoding α subunit of methyl-coenzyme M reductase) sequences confirmed the affiliation of the novel strains with the , and SH was the most closely related species. The 16S rRNA gene and sequence similarities between strains JH1, JH4 and JH8 and SH were 96.2 and 89.0 % respectively, and DNA–DNA hybridization of the isolates and DSM 11976 showed a 20 % reassociation. Strain JH1 exhibited 92 % DNA–DNA relatedness with strains JH4 and JH8, and their 16S rRNA gene and sequences were identical. Cells stained Gram-positive and were non-motile rods, 1.5–1.8 µm long and 0.6 µm wide. The strains were able to use H/CO and formate. The optimum temperature and pH ranges for growth were 37–40 °C and pH 6.5–7.0. The DNA G+C content of strain JH1 was 28 mol%. Based on data from this study using a polyphasic approach, the three strains represent a novel species of genus , for which the name sp. nov. is proposed. The type strain is JH1 ( = KCTC 4102 = JCM 18376).

Funding
This study was supported by the:
  • , National Research Foundation of Korea (NRF) , (Award OGM0211212)
  • , Korea government (MEST)
  • , MEST , (Award NMM0101232 and RBM4351112)
  • , KRIBB Research Initiative Program , (Award KGM4111342 and KCM1051312)
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2013-11-01
2020-10-28
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