1887

Abstract

A taxonomic study was carried out on strain GCS-AN-3, which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3 were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3 was most closely related to DSM 21699 (96.2 %). The principal fatty acids were summed feature 3 (Cω7/Cω6), C, summed feature 8 (Cω7/Cω6) and cyclo C. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed (type strain GCS-AN-3 = LMG 27179 = CGMCC 1.12324 = MCCC 1A01410). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , National Infrastructure of Natural Resources for Science and Technology Program of China , (Award NIMR-2012-9 and NIMR-2011-9)
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2014-03-01
2020-08-15
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