1887

Abstract

A taxonomic study was carried out on strain GCS-AE-31, which was isolated from a phenol-degrading consortium, enriched from coking wastewater activated sludge of the Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AE-31 were Gram-stain-negative, short rods, motile by gliding, oxidase- and catalase-positive. Growth was observed at salinities of 0–3 % and at temperatures of 10–37 °C. On the basis of 16S rRNA gene sequence similarity, strain GCS-AE-31 was most closely related to LMG 10337 (96.17 %), but it showed low similarity to all other species of the genus (89.28–92.45 %). It also showed low 16S rRNA gene similarity to all other species of the family (87.25–92.45 %) examined. The dominant fatty acids were iso-C, summed feature 3 (Cω7/Cω6), anteiso-C and iso-C 3-OH. The menaquinones were MK-7 (95.5 %) and MK-6 (4.5 %). The polar lipids were phosphatidylethanolamine, three aminolipids and three unknown phospholipids. Sphingolipid was present. The G+C content of the chromosomal DNA was 36.2 mol%. According to its phylogenetic position and phenotypic traits, the novel strain could not be assigned to the genus ; it should be classified as representing a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed (type strain GCS-AE-31 = MCCC 1A01299 = CGMCC 1.12329 = LMG 27180). The misclassified species is transferred to the novel genus as comb. nov. (type strain LMG 10337 = MCCC 1A06472 = DSM 12145 = CCUG 39354 = CIP 105500 = JCM 21818 = NBRC 100064).

Funding
This study was supported by the:
  • , National Infrastructure of Natural Resources for Science and Technology Program of China , (Award NIMR-2012-9, NIMR-2013-9 and NIMR-2014-9)
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2014-06-01
2020-10-28
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