1887

Abstract

A novel Gram-negative, aerobic, non-motile and rod-shaped bacterium, designated strain WM67, was isolated from the gut of an abalone () collected from the northern coast of Jeju Island in Korea. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain WM67 clustered in the genus , and the highest sequence similarity was shared with (98.0 % similarity to the type strain). Optimal growth of the isolate occurred at 30 °C, pH 7–8 and with 1 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6) and C. Ubiquinone Q-10 was the major respiratory quinone. The polar lipids of strain WM67 comprised phosphatidylserine, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and three unidentified lipids. The genomic DNA G+C content was 66.5 mol%. DNA–DNA hybridization indicated <17 % genomic relatedness to other members of the genus . The physiological, biochemical, chemotaxonomic and genotypic analyses indicated that strain WM67 represents a novel species of , for which the name sp. nov. is proposed. The type strain is WM67 ( = KACC 17214 = JCM 18872).

Funding
This study was supported by the:
  • Mid-career Researcher Program (Award 2012-0008806)
  • National Research Foundation of Korea
  • National Institute of Biological Resources (NIBR) funded by the Ministry of Environment of the Republic of Korea (Award NIBR no. 2013-02-001)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053892-0
2013-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4626.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053892-0&mimeType=html&fmt=ahah

References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. ( 2005 ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71, 88258835. [View Article] [PubMed]
    [Google Scholar]
  2. Benson H. J. ( 1994 ). Microbiological Applications: a Laboratory Manual in General Microbiology, , 6th edn.. Dubuque, IA:: W. C. Brown;.
    [Google Scholar]
  3. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. ( 2008 ). Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75, 523530. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D. ( 1981a ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316354.[PubMed]
    [Google Scholar]
  5. Collins M. D., Jones D. ( 1981b ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51, 129134. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  8. Goszczynska T., Serfontein J. J. ( 1998 ). Milk-Tween agar, a semiselective medium for isolation and differentiation of Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv. phaseoli. . J Microbiol Methods 32, 6572. [View Article]
    [Google Scholar]
  9. Jung Y. T., Kim B. H., Oh T. K., Yoon J. H. ( 2010 ). Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria . . Int J Syst Evol Microbiol 60, 11771181. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [View Article]
    [Google Scholar]
  12. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  13. MIDI ( 1999 ). Sherlock Microbial Identification System Operating Manual, , version 3.0.. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  14. Oakes F. R., Ponte R. D. ( 1996 ). The abalone market: opportunities for cultured abalone. . Aquaculture 140, 187195. [View Article]
    [Google Scholar]
  15. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. ( 1992 ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79, 5965.[PubMed] [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  18. Sawabe T., Oda Y., Shiomi Y., Ezura Y. ( 1995 ). Alginate degradation by bacteria isolated from the gut of sea-urchins and abalones. . Microb Ecol 30, 193202. [View Article]
    [Google Scholar]
  19. Sawabe T., Inoue S., Fukui Y., Yoshie K., Nishihara Y., Miura H. ( 2007 ). Mass mortality of Japanese abalone Haliotis discus hannai caused by Vibrio harveyi infection. . Microbes Environ 22, 300308. [View Article]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  21. Tanaka R., Sugimura I., Sawabe T., Yoshimizu M., Ezura Y. ( 2003 ). Gut microflora of abalone Haliotis discus hannai in culture changes coincident with a change in diet. . Fish Sci 69, 951958. [View Article]
    [Google Scholar]
  22. Tanaka R., Ootsubo M., Sawabe T., Ezura Y., Tajima K. ( 2004 ). Biodiversity and in situ abundance of gut microflora of abalone (Haliotis discus hannai) determined by culture-independent techniques. . Aquaculture 241, 453463. [View Article]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  24. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  25. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575580.[PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  27. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. ( 2000 ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. . Int J Syst Evol Microbiol 50, 12971303. [View Article] [PubMed]
    [Google Scholar]
  28. Yamamoto S., Harayama S. ( 1995 ). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. . Appl Environ Microbiol 61, 3768.[PubMed]
    [Google Scholar]
  29. Yoon J. H., Lee S. Y., Kang S. J., Lee C. H., Oh T. K. ( 2007 ). Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57, 542547. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053892-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053892-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error