Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant
Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21T exhibited the highest sequence similarity to Bosea minatitlanensis AMX51T (93.43 %) and Bosea thiooxidans DSM 9653T (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18 : 1ω7c and 11-methyl C18 : 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21T was 68.9 mol%. A new genus and species, Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21T ( = DSM 25521T = NCAIM B 02510T) is the type strain of Phreatobacter oligotrophus.
AmannR.,
SnaidrJ.,
WagnerM.,
LudwigW.,
SchleiferK.-H.(1996). In situ visualization of high genetic diversity in a natural microbial community. . J Bacteriol178, 3496–3500. [PubMed]
BohusV.,
TóthE. M.,
SzékelyA. J.,
MakkJ.,
BaranyiK.,
PatekG.,
SchunkJ.,
MárialigetiK.(2010). Microbiological investigation of an industrial ultra pure supply water plant using cultivation-based and cultivation-independent methods. . Water Res44, 6124–6132. [View Article] [PubMed]
BrennerD. J.,
HollisD. G.,
MossC. W.,
EnglishC. K.,
HallG. S.,
VincentJ.,
RadosevicJ.,
BirknessK. A.,
BibbW. F.& other authors (1991). Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies. . J Clin Microbiol29, 2450–2460. [PubMed]
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.(1977). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem81, 461–466. [View Article] [PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100, 221–230. [View Article] [PubMed]
CostertonJ. W.,
ChengK.-J.,
GeeseyG. G.,
LaddT. I. M.,
NickelJ. C.,
DasguptaM.,
MarrieT. J.(1987). Bacterial biofilms in nature and disease. . Annu Rev Microbiol41, 435–464. [View Article] [PubMed]
DasS. K.,
MishraA. K.,
TindallB. J.,
RaineyF. A.,
StackebrandtE.(1996). Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. . Int J Syst Bacteriol46, 981–987. [View Article] [PubMed]
De LeyJ.,
CattoirH.,
ReynaertsA.(1970). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12, 133–142. [View Article] [PubMed]
EmbleyT. M.,
WaitR.(1994). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 141–147. Edited by
GoodfellowM.,
O’DonnellA. G.
. New York:: Wiley;.
EmersonD. R.,
WordenR. M.,
BreznakJ. A.(1994). A diffusion gradient chamber for studying microbial behavior and separating microorganisms. . Appl Environ Microbiol60, 1269–1278. [PubMed]
GrothI.,
SchumannP.,
RaineyF. A.,
MartinK.,
SchuetzeB.,
AugstenK.(1997).Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol47, 1129–1133. [View Article] [PubMed]
HasegawaT.,
TakizawaM.,
TanidaS.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol29, 319–322. [View Article]
HughR.,
LeifsonE.(1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. . J Bacteriol66, 24–26. [PubMed]
HussV. A. R.,
FestlH.,
SchleiferK. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol4, 184–192. [View Article] [PubMed]
KékiZs.,
GrébnerK.,
BohusV.,
MárialigetiK.,
TóthE. M.(2013). Application of special oligotrophic media for cultivation of bacterial communities originated from ultrapure water. . Acta Microbiol Immunol Hung60, 345–357. [View Article] [PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article] [PubMed]
La ScolaB.,
MalletM. N.,
GrimontP. A.,
RaoultD.(2002). Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A. . Int J Syst Evol Microbiol52, 1773–1782. [View Article] [PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol47, 87–95. [View Article]
PattersonM. K.,
HustedG. R.,
RutkowskiA.,
MayetteD. C.(1991). Isolation, identification and microscopic properties of biofilms in high-purity water distribution systems. . Ultrapure Water8, 18–23.
ReasonerD. J.,
GeldreichE. E.(1985). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol49, 1–7. [PubMed]
SmibertR. M.,
KriegN. R.(1994). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
SoiniS. M.,
KoskinenK. T.,
VileniusM. J.,
PuhakkaJ. A.(2002). Occurrence of bacteria in industrial fluid power systems. . Clean Technol Environ Policy4, 26–31. [View Article]
SteadD. E.,
SellwoodJ. E.,
WilsonJ.,
VineyI.(1992). Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. . J Appl Microbiol72, 315–321. [View Article]
StevensonB. S.,
EichorstS. A.,
WertzJ. T.,
SchmidtT. M.,
BreznakJ. A.(2004). New strategies for cultivation and detection of previously uncultured microbes. . Appl Environ Microbiol70, 4748–4755. [View Article] [PubMed]
TamakiH.,
SekiguchiY.,
HanadaS.,
NakamuraK.,
NomuraN.,
MatsumuraM.,
KamagataY.(2005). Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. . Appl Environ Microbiol71, 2162–2169. [View Article] [PubMed]
TamaokaJ.,
KomagataK.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25, 125–128. [View Article]
TóthE. M.,
SchumannP.,
BorsodiA. K.,
KékiZ.,
KovácsA. L.,
MárialigetiK.(2008).Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). . Int J Syst Evol Microbiol58, 976–981. [View Article] [PubMed]
TóthE. M.,
KékiZs.,
BohusV.,
BorsodiA. K.,
MárialigetiK.,
SchumannP.(2012).Aquipuribacter hungaricus gen. nov., sp. nov., an actinobacterium isolated from the ultrapure water system of a power plant. . Int J Syst Evol Microbiol62, 556–562. [View Article] [PubMed]
Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant