1887

Abstract

A bacterial strain, designated 5N26, was isolated from an agricultural soil cultivated with Chinese cabbage (). Cells of this strain were Gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. The major fatty acids of strain 5N26 were C (7.5 %), C 2-OH (13.4 %) and summed feature 8 (Cω6 and/or Cω7; 63.2 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The G+C content of the genomic DNA of strain 5N26 was 68.3 mol%. 16S rRNA gene sequence analysis showed that strain 5N26 was phylogenetically related to TH-G33 and DS-48 (97.0 % and 96.6 % sequence similarity, respectively). The results of genotypic and phenotypic data showed that strain 5N26 could be distinguished from phylogenetically related species, and that this strain represented a novel species within the genus , for which the name sp. nov. (type strain 5N26 = KACC 16376 = NBRC 109097) is proposed.

Funding
This study was supported by the:
  • Rural Development Administration, Republic of Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053827-0
2014-03-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/1024.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053827-0&mimeType=html&fmt=ahah

References

  1. Baik K. S., Park S. C., Choe H. N., Kim S. N., Moon J. H., Seong C. N. ( 2012 ). Roseomonas riguiloci sp. nov., isolated from wetland freshwater. . Int J Syst Evol Microbiol 62, 30243029. [View Article] [PubMed]
    [Google Scholar]
  2. Baker G. C., Smith J. J., Cowan D. A. ( 2003 ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55, 541555. [View Article] [PubMed]
    [Google Scholar]
  3. Bard J. D., Deville J. G., Summanen P. H., Lewinski M. A. ( 2010 ). Roseomonas mucosa isolated from bloodstream of pediatric patient. . J Clin Microbiol 48, 30273029. [View Article] [PubMed]
    [Google Scholar]
  4. Bibashi E., Sofianou D., Kontopoulou K., Mitsopoulos E., Kokolina E. ( 2000 ). Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. . J Clin Microbiol 38, 456457.[PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution – minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  8. Furuhata K., Miyamoto H., Goto K., Kato Y., Hara M., Fukuyama M. ( 2008 ). Roseomonas stagni sp. nov., isolated from pond water in Japan. . J Gen Appl Microbiol 54, 167171. [View Article] [PubMed]
    [Google Scholar]
  9. Gallego V., Sánchez-Porro C., García M. T., Ventosa A. ( 2006 ). Roseomonas aquatica sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 56, 22912295. [View Article] [PubMed]
    [Google Scholar]
  10. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [View Article] [PubMed]
    [Google Scholar]
  11. Han X. Y., Pham A. S., Tarrand J. J., Rolston K. V., Helsel L. O., Levett P. N. ( 2003 ). Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. . Am J Clin Pathol 120, 256264. [View Article] [PubMed]
    [Google Scholar]
  12. Islam M. R., Sultana T., Cho J. C., Joe M. M., Sa T. M. ( 2012 ). Diversity of free-living nitrogen-fixing bacteria associated with Korean paddy fields. . Ann Microbiol 62, 16431650. [View Article]
    [Google Scholar]
  13. Jiang C. Y., Dai X., Wang B. J., Zhou Y. G., Liu S. J. ( 2006 ). Roseomonas lacus sp. nov., isolated from freshwater lake sediment. . Int J Syst Evol Microbiol 56, 2528. [View Article] [PubMed]
    [Google Scholar]
  14. Kim M. S., Baik K. S., Park S. C., Rhee M. S., Oh H. M., Seong C. N. ( 2009 ). Roseomonas frigidaquae sp. nov., isolated from a water-cooling system. . Int J Syst Evol Microbiol 59, 16301634. [View Article] [PubMed]
    [Google Scholar]
  15. Kim S. J., Weon H. Y., Ahn J. H., Hong S. B., Seok S. J., Whang K. S., Kwon S. W. ( 2013 ). Roseomonas aerophila sp. nov., isolated from air. . Int J Syst Evol Microbiol 63, 23342337. [View Article] [PubMed]
    [Google Scholar]
  16. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  17. Lee S., Malone C., Kemp P. ( 1993 ). Use of multiple 16S rRNA-targeted fluorescent probes to increase signal strength and measure cellular RNA from natural planktonic bacteria. . Mar Ecol Prog Ser 101, 193201. [View Article]
    [Google Scholar]
  18. Lopes A., Esperito Santo C., Grass G., Chung A. P., Morais P. V. ( 2011 ). Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin. . Int J Syst Evol Microbiol 61, 610615. [View Article] [PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+ C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Evol Microbiol 39, 159167. [View Article]
    [Google Scholar]
  20. MIDI ( 1999 ). Sherlock Microbial Identification System, Operating Manual, version 3.0. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  21. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L. ( 1993 ). Roseomonas, a new genus associated with bacteremia and other human infections. . J Clin Microbiol 31, 32753283.[PubMed]
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  23. Sánchez-Porro C., Gallego V., Busse H. J., Kämpfer P., Ventosa A. ( 2009 ). Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas . . Int J Syst Evol Microbiol 59, 11931198. [View Article] [PubMed]
    [Google Scholar]
  24. Sandoe J. A., Malnick H., Loudon K. W. ( 1997 ). A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. . J Clin Microbiol 35, 21502152.[PubMed]
    [Google Scholar]
  25. Sfanos K., Harmody D., Dang P., Ledger A., Pomponi S., McCarthy P., Lopez J. ( 2005 ). A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates. . Syst Appl Microbiol 28, 242264. [View Article] [PubMed]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Subudhi C. P., Adedeji A., Kaufmann M. E., Lucas G. S., Kerr J. R. ( 2001 ). Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. . Clin Microbiol Infect 7, 573575. [View Article] [PubMed]
    [Google Scholar]
  28. Tamaki H., Sekiguchi Y., Hanada S., Nakamura K., Nomura N., Matsumura M., Kamagata Y. ( 2005 ). Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. . Appl Environ Microbiol 71, 21622169. [View Article] [PubMed]
    [Google Scholar]
  29. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  30. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  31. Venkata Ramana V., Sasikala Ch., Takaichi S., Ramana Ch. V. ( 2010 ). Roseomonas aestuarii sp. nov., a bacteriochlorophyll-a containing alphaproteobacterium isolated from an estuarine habitat of India. . Syst Appl Microbiol 33, 198203. [View Article] [PubMed]
    [Google Scholar]
  32. Yoo S. H., Weon H. Y., Noh H. J., Hong S. B., Lee C. M., Kim B. Y., Kwon S. W., Go S. J. ( 2008 ). Roseomonas aerilata sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 58, 14821485. [View Article] [PubMed]
    [Google Scholar]
  33. Yoon J. H., Kang S. J., Oh H. W., Oh T. K. ( 2007 ). Roseomonas terrae sp. nov.. Int J Syst Evol Microbiol 57, 24852488. [View Article] [PubMed]
    [Google Scholar]
  34. Zhang Y. Q., Yu L. Y., Wang D., Liu H. Y., Sun C. H., Jiang W., Zhang Y. Q., Li W. J. ( 2008 ). Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. . Int J Syst Evol Microbiol 58, 20702074. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053827-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053827-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error