1887

Abstract

A taxonomic study employing a polyphasic approach was performed on a novel anaerobic bacterium isolated from natural gas production-water. The bacterium stained Gram-negative and consisted of non-motile, non-spore-forming, rod-shaped cells. Products of glucose or starch fermentation were ethanol, CO, formate, acetate and H2. The predominant fatty acids were C ALDE and summed feature 3 comprising Cω7 and/or Cω6. The DNA G+C content was 45.5 mol%. 16S rRNA gene sequence analysis demonstrated that the nearest phylogenetic neighbours of the novel strain were DSM 6139 (98.5 %) and JCM 14817 (95.4 %). The DNA–DNA hybridization value between the novel organism and PeC1 DSM 6139 was determined to be only 30.2 %, demonstrating the separateness of the two species. Based on phylogenetic, phenotypic and chemotaxonomic evidence that clearly distinguished strain 232.1 from and other close relatives, it is proposed that the novel isolate be classified as representing a novel species of a new genus within the family , gen. nov., sp. nov. The type strain of the type species is 232.1 ( = ATCC BAA-2257 = DSM 24749). In addition, is proposed to be reclassified as comb. nov.

Funding
This study was supported by the:
  • Conoco Philips Petroleum
  • National Science Foundation (Award OCE 0961900 and MCB-0921265)
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2014-01-01
2024-03-29
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