1887

Abstract

A Gram-stain-negative, aerobic, motile by gliding, non-spore-forming, rod-shaped, orange-pigmented bacterium, designated strain H32-4, was isolated from 32 m deep water of Daechung reservoir in Daejeon, Republic of Korea. Based on the nucleotide sequence of the 16S rRNA gene, the closest neighbouring type strain was NJ-8 with which strain H32-4 shared 98.9 % sequence similarity. The most abundant fatty acids in whole cells of strain H32-4 were C iso (40.6 %), C iso 3-OH (22.4 %), summed feature 3 (Cω7 and/or Cω6; 9.3 %) and C (6.6 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA of strain H32-4 was 45.7 mol%. Thus, these combined genotypic and phenotypic data supported the conclusion that strain H32-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H32-4 ( = KCTC 32395 = JCM 19172).

Funding
This study was supported by the:
  • , Advanced Biomass R&D Centre
  • , Global Frontier Program
  • , Korean Ministry of Education, Science & Technology
  • , CAER (Centre for Aquatic Ecosystem Restoration)
  • , Korean Ministry of Environment
  • , KIST Institutional Program , (Award 2E24280)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053702-0
2013-12-01
2020-07-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4519.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053702-0&mimeType=html&fmt=ahah

References

  1. Bates R. G., Bower V. E. ( 1956 ). Alkaline solutions for pH control. . Anal Chem 28, 13221324. [CrossRef]
    [Google Scholar]
  2. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1, 138146. [CrossRef]
    [Google Scholar]
  6. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucl Acids Symp Ser 41, 9598.
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  8. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  9. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299306. [CrossRef] [PubMed]
    [Google Scholar]
  10. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  11. Qu J.-H., Yuan H.-L., Yang J.-S., Li H.-F., Chen N. ( 2009 ). Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. . Int J Syst Evol Microbiol 59, 11531157. [CrossRef] [PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  13. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
  14. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [CrossRef]
    [Google Scholar]
  15. Tarrand J. J., Gröschel D. H. M. ( 1982 ). Rapid, modified oxidase test for oxidase-variable bacterial isolates. . J Clin Microbiol 16, 772774.[PubMed]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053702-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053702-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error