1887

Abstract

Two strains (NYNU 121010 and NYNU 121032) of a novel basidiomycetous yeast species belonging to the genus were isolated from insect frass collected from trunks of a pagoda tree ( L.) in Yantai, Shandong province, east China. The sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the closest relatives were FIRDI 007, CBS 7429 and sp. S6A. The D1/D2 sequences of the novel strains differed by 12 nt substitutions (2 %) from the type strain of . , and by 13 nt substitutions (2.2 %) from the type strain of . and from sp. S6A. The novel strains differed from closely related species by more than 4.6 % substitutions in the ITS region. The novel strains can also be distinguished from . and . on the basis of a number of morphological and physiological characteristics and represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NYNU 121010 ( = CICC 32998 = CBS 12813). The Mycobank deposit number is MB 804119.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053686-0
2013-09-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3501.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053686-0&mimeType=html&fmt=ahah

References

  1. Begerow D., Bauer R., Oberwinkler F.. ( 2001;). Muribasidiospora: Microstromatales or Exobasidiales?. Mycol Res 105:, 798–810. [CrossRef]
    [Google Scholar]
  2. Begerow D., Stoll M., Bauer R.. ( 2006;). A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. . Mycologia 98:, 906–916. [CrossRef][PubMed]
    [Google Scholar]
  3. de Beer Z. W., Begerow D., Bauer R., Pegg G. S., Crous P. W., Wingfield M. J.. ( 2006;). Phylogeny of the Quambalariaceae fam. nov., including important Eucalyptus pathogens in South Africa and Australia. . Stud Mycol 55:, 289–298. [CrossRef][PubMed]
    [Google Scholar]
  4. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A.. ( 2000;). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. . Int J Syst Evol Microbiol 50:, 1351–1371. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Golubev V. I., Kulakovskaia T. V., Kulakovskaia E. V., Golubev N. V.. ( 2004;). [ The fungicidal activity of an extracellular glycolipid from Sympodiomycopsis paphiopedili Sugiyama et al.. ]. Mikrobiologiia 73:, 841–845 (in Russian).[PubMed]
    [Google Scholar]
  7. Hamamoto M., Nagahama T., Tamura M.. ( 2002;). Systematic study of basidiomycetous yeasts - evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium. . FEMS Yeast Res 2:, 409–413.[PubMed]
    [Google Scholar]
  8. Jindamorakot S., Am-In S., Thuy T. T., Duy N. D., Kawasaki H., Potacharoen W., Limtong S., Tanticharoen M., Nakase T.. ( 2004;). Candida easanensis sp. nov., Candida pattaniensis sp. nov. and Candida nakhonratchasimensis sp. nov., three new species of yeasts isolated from insect frass in Thailand. . J Gen Appl Microbiol 50:, 261–269. [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  10. Kulakovskaya T. V., Shashkov A. S., Kulakovskaya E. V., Golubev W. I.. ( 2004;). Characterization of an antifungal glycolipid secreted by the yeast Sympodiomycopsis paphiopedili. . FEMS Yeast Res 5:, 247–252. [CrossRef][PubMed]
    [Google Scholar]
  11. Kurtzman C. P., Robnett C. J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef][PubMed]
    [Google Scholar]
  12. Mahdi L. E., Statzell-Tallman A., Fell J. W., Brown M. V., Donachie S. P.. ( 2008;). Sympodiomycopsis lanaiensis sp. nov., a basidiomycetous yeast (Ustilaginomycotina: Microstromatales) from marine driftwood in Hawai’i. . FEMS Yeast Res 8:, 1357–1363. [CrossRef][PubMed]
    [Google Scholar]
  13. Nakase T., Suzuki M.. ( 1986;). Bullera megalospora, a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. . J Gen Appl Microbiol 32:, 225–240. [CrossRef]
    [Google Scholar]
  14. Ramos J. P., Valente P., De Souza R. A., Rosa C. A., Leoncini O.. ( 2001;). Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. . Lett Åppl Microbiol 33:, 206–210. [CrossRef][PubMed]
    [Google Scholar]
  15. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  16. Sipiczki M., Kajdacsi E.. ( 2009;). Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. . Int J Syst Evol Microbiol 59:, 914–920. [CrossRef][PubMed]
    [Google Scholar]
  17. Sugiyama J., Tokuoka K., Suh S.-O., Hirata A., Komagata K.. ( 1991;). Sympodiomycopsis: a new yeast-like anamorph genus with basidiomycetous nature from orchid nectar. . Antonie van Leeuwenhoek 59:, 95–108. [CrossRef][PubMed]
    [Google Scholar]
  18. Thanh V. N., Hai D. A., Lachance M. A.. ( 2003;). Issatchenkia hanoiensis, a new yeast species isolated from frass of the litchi fruit borer Conopomorpha cramerella Snellen. . FEMS Yeast Res 4:, 113–117. [CrossRef][PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  20. Wei Y. H., Liou G. Y., Liu H. Y., Lee F. L.. ( 2011;). Sympodiomycopsis kandeliae sp. nov., a basidiomycetous anamorphic fungus from mangroves, and reclassification of Sympodiomycopsis lanaiensis as Jaminaea lanaiensis comb. nov.. Int J Syst Evol Microbiol 61:, 469–473. [CrossRef][PubMed]
    [Google Scholar]
  21. White T. J., Bruns T., Lee S., Taylor J.. ( 1990;). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Applications, pp. 315–322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J... San Diego, CA:: Academic Press;.
    [Google Scholar]
  22. Yamada Y., Kondo K.. ( 1973;). Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium. . J Gen Appl Microbiol 19:, 59–77. [CrossRef]
    [Google Scholar]
  23. Yarrow D.. ( 1998;). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P., Fell J. W... Amsterdam:: Elsevier;. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053686-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053686-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error