1887

Abstract

A Gram-stain-negative, strictly aerobic, slightly halophilic, non-motile and rod-shaped bacterial strain, designated P2E16, was isolated from mangrove () rhizosphere, collected at Devipattinam mangroves, Tamil Nadu, India. Strain P2E16 grew optimally at pH 7.0–8.0, at 25–28 °C and in the presence of 2–3 % (w/v) NaCl. 16S rRNA gene analysis showed that strain P2E16 was phylogenetically closely related to the genus , with SM-A87 as the closest related type strain (98.2 % 16S rRNA gene sequence similarity) and less than 93 % 16S rRNA gene sequence similarity to all other members of the family . Strain P2E16 contained MK-6 as the major respiratory quinone, phosphatidylethanolamine as the predominant polar lipid and iso-C (17.8 %), iso-C 3-OH (15.1 %), C (12.8 %), iso-Cω9 (9.8 %), iso-C G (9.0 %), and summed feature 3 (comprising Cω7 and/or iso-C 2-OH; 7.1 %) as the major fatty acids. The DNA G+C content was 34.3 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness and low DNA–DNA relatedness demonstrated that strain P2E16 was distinct from the type strain of . On the basis of these presented data, strain P2E16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P2E16 ( = DSM 24499 = LMG 26237 = KCTC 23496). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Council of Scientific and Industrial research (CSIR), Government of India (Award BSC0117)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053512-0
2014-02-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/545.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053512-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  2. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. ( 1988 ). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. . J Clin Microbiol 26, 17081713.[PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 11291133. [View Article] [PubMed]
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  12. Montero-Calasanz M. D., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M. ( 2013 ). Chryseobacterium hispalensesp. nov., a plant growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery and emendation of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. . Int J Syst Evol Microbiol 63, 43864395. [View Article] [PubMed]
    [Google Scholar]
  13. Qin Q. L., Zhao D. L., Wang J., Chen X. L., Dang H. Y., Li T. G., Zhang Y. Z., Gao P. J. ( 2007a ). Wangia profunda gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from southern Okinawa Trough deep-sea sediment. . FEMS Microbiol Lett 271, 5358. [View Article] [PubMed]
    [Google Scholar]
  14. Qin Q. L., Zhao D. L., Wang J., Chen X. L., Dang H. Y., Li T. G., Zhang Y. Z., Gao P. J. ( 2007b ). Zunongwangia, gen nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 116. . Int J Syst Evol Microbiol 57, 13711373. [View Article] [PubMed]
    [Google Scholar]
  15. Rameshkumar N., Nair S. ( 2009 ). Isolation and molecular characterization of genetically diverse antagonistic, diazotrophic red-pigmented vibrios from different mangrove rhizospheres. . FEMS Microbiol Ecol 67, 455467. [View Article] [PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20, 16.
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  19. Stanier R. Y., Palleroni N. J., Doudoroff M. ( 1966 ). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43, 159271. [View Article] [PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0.. Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.. Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. A., Kreig N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.053512-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053512-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error