1887

Abstract

A Gram-stain-negative, strictly aerobic, slightly halophilic, non-motile and rod-shaped bacterial strain, designated P2E16, was isolated from mangrove () rhizosphere, collected at Devipattinam mangroves, Tamil Nadu, India. Strain P2E16 grew optimally at pH 7.0–8.0, at 25–28 °C and in the presence of 2–3 % (w/v) NaCl. 16S rRNA gene analysis showed that strain P2E16 was phylogenetically closely related to the genus , with SM-A87 as the closest related type strain (98.2 % 16S rRNA gene sequence similarity) and less than 93 % 16S rRNA gene sequence similarity to all other members of the family . Strain P2E16 contained MK-6 as the major respiratory quinone, phosphatidylethanolamine as the predominant polar lipid and iso-C (17.8 %), iso-C 3-OH (15.1 %), C (12.8 %), iso-Cω9 (9.8 %), iso-C G (9.0 %), and summed feature 3 (comprising Cω7 and/or iso-C 2-OH; 7.1 %) as the major fatty acids. The DNA G+C content was 34.3 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness and low DNA–DNA relatedness demonstrated that strain P2E16 was distinct from the type strain of . On the basis of these presented data, strain P2E16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P2E16 ( = DSM 24499 = LMG 26237 = KCTC 23496). An emended description of the genus is also provided.

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2014-02-01
2019-10-22
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