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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_4

gen. nov. in the family with two novel species, sp. nov. and sp. nov., and reclassification of as gen. nov., comb. nov. Free

Abstract

Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026 and MEBiC 08903, were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with DSM 9555. 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA–DNA relatedness between the two were 31.3–31.8 %. Phylogenetic analysis revealed that the two isolates and DSM 9555 were affiliated with the family in the class . The dominant fatty acids of strains MEBiC 07026, MEBiC 08903 and DSM 9555 were branched-type or hydroxylated C, but DSM 9555 contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family . The major respiratory quinone of the strains was MK-7. Strains MEBiC07026 and MEBiC08903 utilized a wide range of carboxylic acids whereas DSM 9555 utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of DSM 9555 revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, gen. nov., is proposed in the family with two novel species, sp. nov. with type strain MEBiC 07026 ( = KCCM 42978 = JCM 18290) and sp. nov. with type strain MEBiC 08903 ( = KCCM 43024 = JCM 19490). Additionally, DSM 9555 ( = ATCC 19072) is reclassified as gen. nov., comb. nov.

  • Published Online:
Funding
This study was supported by the:
  • MBRB
  • Ministry of Ocean & Fisheries, Korea
  • KRCF
  • KIST
  • KIOST
© 2014 IUMS
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2014-04-01
2025-01-16
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Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga fermentans as Saccharicrinis fermentans gen. nov., comb. nov.
Int J Syst Evol Microbiol 64, 1351 (2014); https://doi.org/10.1099/ijs.0.053462-0
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