1887

Abstract

Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026 and MEBiC 08903, were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with [] DSM 9555. 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA–DNA relatedness between the two were 31.3–31.8 %. Phylogenetic analysis revealed that the two isolates and [] DSM 9555 were affiliated with the family in the class . The dominant fatty acids of strains MEBiC 07026, MEBiC 08903 and [] DSM 9555 were branched-type or hydroxylated C, but [] DSM 9555 contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family . The major respiratory quinone of the strains was MK-7. Strains MEBiC07026 and MEBiC08903 utilized a wide range of carboxylic acids whereas [] DSM 9555 utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of [] DSM 9555 revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, gen. nov., is proposed in the family with two novel species, sp. nov. with type strain MEBiC 07026 ( = KCCM 42978 = JCM 18290) and sp. nov. with type strain MEBiC 08903 ( = KCCM 43024 = JCM 19490). Additionally, [] DSM 9555 ( = ATCC 19072) is reclassified as gen. nov., comb. nov.

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2014-04-01
2019-12-13
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