1887

Abstract

Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026 and MEBiC 08903, were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with DSM 9555. 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA–DNA relatedness between the two were 31.3–31.8 %. Phylogenetic analysis revealed that the two isolates and DSM 9555 were affiliated with the family in the class . The dominant fatty acids of strains MEBiC 07026, MEBiC 08903 and DSM 9555 were branched-type or hydroxylated C, but DSM 9555 contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family . The major respiratory quinone of the strains was MK-7. Strains MEBiC07026 and MEBiC08903 utilized a wide range of carboxylic acids whereas DSM 9555 utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of DSM 9555 revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, gen. nov., is proposed in the family with two novel species, sp. nov. with type strain MEBiC 07026 ( = KCCM 42978 = JCM 18290) and sp. nov. with type strain MEBiC 08903 ( = KCCM 43024 = JCM 19490). Additionally, DSM 9555 ( = ATCC 19072) is reclassified as gen. nov., comb. nov.

Funding
This study was supported by the:
  • MBRB
  • Ministry of Ocean & Fisheries, Korea
  • KRCF
  • KIST
  • KIOST
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053462-0
2014-04-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1351.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053462-0&mimeType=html&fmt=ahah

References

  1. Bachmann B. J. ( 1955 ). Studies on Cytophaga fermentans, n.sp., a facultatively anaerobic lower myxobacterium. . J Gen Microbiol 13, 541551. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. Denger K., Warthmann R., Ludwig W., Schink B. ( 2002 ). Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium. . Int J Syst Evol Microbiol 52, 173178.[PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406. [View Article]
    [Google Scholar]
  6. Gao Z.-M., Liu X., Zhang X.-Y., Ruan L.-W. ( 2013 ). Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment. . Int J Syst Evol Microbiol 63, 109113. [View Article] [PubMed]
    [Google Scholar]
  7. Haack S. K., Breznak J. A. ( 1993 ). Cytophaga xylanolytica sp. nov., a xylan-degrading, anaerobic gliding bacterium. . Arch Microbiol 159, 615. [View Article]
    [Google Scholar]
  8. Huang X.-F., Liu Y. J., Dong J.-D., Qu L.-Y., Zhang Y.-Y., Wang F.-Z., Tian X.-P., Zhang S. ( 2013 ). Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. . Int J Syst Evol Microbiol doi:10.1099/ijs.0.052779-0. [View Article]
    [Google Scholar]
  9. Kaneko T., Nozaki R., Aizawa K. ( 1978 ). Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis . . Microbiol Immunol 22, 639641. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Kwon K. K., Lee H.-S., Yang S. H., Kim S.-J. ( 2005 ). Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. . Int J Syst Evol Microbiol 55, 20332037. [View Article] [PubMed]
    [Google Scholar]
  12. Ludwig W., Euzéby J., Whitman W. B. ( 2011 ). Family II. Marinilabiliaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, p. 49. Edited by Krieg N. R., James T. S., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  13. Minnkin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipid. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  14. Miyazaki M., Koide O., Kobayashi T., Mori K., Shimamura S., Nunoura T., Imachi H., Inagaki F., Nagahama T. & other authors ( 2012 ). Geofilum rubicundum gen. nov., sp. nov., isolated from deep subseafloor sediment. . Int J Syst Evol Microbiol 62, 10751080. [View Article] [PubMed]
    [Google Scholar]
  15. Nakagawa Y. ( 2011 ). Genus I. Cytophaga . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 371375. Edited by Krieg N. R., James T. S., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  16. Nakagawa Y., Yamasato K. ( 1993 ). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. . J Gen Microbiol 139, 11551161. [View Article] [PubMed]
    [Google Scholar]
  17. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  18. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Sohn J. H., Kwon K. K., Kang J.-H., Jung H.-B., Kim S.-J. ( 2004 ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. . Int J Syst Evol Microbiol 54, 14831487. [View Article] [PubMed]
    [Google Scholar]
  21. Sorokin D. Y., Panteleeva A. N., Tourova T. P., Kaparullina E. N., Muyzer G. ( 2011 ). Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes. . Extremophiles 15, 691696. [View Article] [PubMed]
    [Google Scholar]
  22. Stackebrandt E., Liesack W. ( 1993 ). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 158160. Edited by Goodfellow M., O’Donnell A. G. . London:: Academic Press;.
    [Google Scholar]
  23. Suzuki M. ( 2011 ). Genus I. Marinilabilia . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 4951. Edited by Krieg N. R., James T. S., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  24. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 1999 ). Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. . Int J Syst Bacteriol 49, 15511557. [View Article] [PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  27. Yang S.-H., Kwon K. K., Lee H.-S., Kim S.-J. ( 2006 ). Shewanella spongiae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 56, 28792882. [View Article] [PubMed]
    [Google Scholar]
  28. Yang S.-H., Seo H.-S., Oh H.-M., Kim S.-J., Lee J.-H., Kwon K. K. ( 2013 ). Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale . . Int J Syst Evol Microbiol 63, 11051110. [View Article] [PubMed]
    [Google Scholar]
  29. Zhao B., Chen S. ( 2012 ). Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake. . Int J Syst Evol Microbiol 62, 26182623. [View Article] [PubMed]
    [Google Scholar]
  30. Zhao C., Gao Z., Qin Q., Ruan L. ( 2012 ). Mangroviflexus xiamenensis gen. nov., sp. nov., a member of the family Marinilabiliaceae isolated from mangrove sediment. . Int J Syst Evol Microbiol 62, 18191824. [View Article] [PubMed]
    [Google Scholar]
  31. Zhilina T. N., Appel R., Probian C., Brossa E. L., Harder J., Widdel F., Zavarzin G. A. ( 2004 ). Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake. . Arch Microbiol 182, 244253. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053462-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053462-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error