1887

Abstract

Two strains, designated GCR0103 and GYR3121, were isolated from seawater of the Saemangeum Embankment in Jeollabuk-do, Korea. The cells of the two strains were Gram-reaction-negative and non-motile, and formed multicellular filaments. The colonies of the two strains were pink-pigmented and able to grow at 15–37 °C (optimum 25 °C) on R2A and NA medium. Strains GCR0103 and GYR3121 grew at pH 6.5–10 (optimum pH 7.5) and pH 5.5–9.5 (optimum pH 7.5), and within NaCl ranges of 0–0.4 % and 0–1 %, respectively. The polar lipid profiles of the two strains contained phosphatidylethanolamine, five unknown aminolipids, an unknown phospholipid and four or five unknown lipids. The DNA G+C contents of strains GCR0103 and GYR3121 were 56.0 and 54.5 mol%, respectively. The respiratory quinone detected in both strains was MK-7. The 16S rRNA gene sequence similarity between GCR0103 and GYR3121 was 95.5 %. The 16S rRNA gene sequence similarities of the two strains to closely related reference strains were less than 89 %. Phylogenetic analysis based on 16S rRNA genes showed that GCR0103 and GYR3121 formed a distinct phyletic line in the family . On the basis of the phenotypic, chemotaxonomic and phylogenetic properties, strains GCR0103 and GYR3121 represent two novel species in a new genus within the family , for which the names gen. nov., sp. nov. and sp. nov. are proposed. The type strain of is GCR0103 ( = KACC 16453 = JCM 17927) and the type strain of is GYR3121 ( = KACC 16447 = JCM 17925).

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR), Korea
  • , Joint Degree and Research Center for Biorefinery
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2013-12-01
2020-09-25
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