RT Journal Article SR Electronic(1) A1 Gu, Chun Tao A1 Li, Chun Yan A1 Yang, Li Jie A1 Huo, Gui ChengYR 2013 T1 Lactobacillus heilongjiangensis sp. nov., isolated from Chinese pickle JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_11 SP 4094 OP 4099 DO https://doi.org/10.1099/ijs.0.053355-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive bacterial strain, S4-3T, was isolated from traditional pickle in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, dnaK gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA–DNA hybridization and an analysis of phenotypic features. Strain S4-3T showed 97.9–98.7 % 16S rRNA gene sequence similarities, 84.4–94.1 % pheS gene sequence similarities and 94.4–96.9 % rpoA gene sequence similarities to the type strains of Lactobacillus nantensis , Lactobacillus mindensis , Lactobacillus crustorum , Lactobacillus futsaii , Lactobacillus farciminis and Lactobacillus kimchiensis . dnaK gene sequence similarities between S4-3T and Lactobacillus nantensis LMG 23510T, Lactobacillus mindensis LMG 21932T, Lactobacillus crustorum LMG 23699T, Lactobacillus futsaii JCM 17355T and Lactobacillus farciminis LMG 9200T were 95.4, 91.5, 90.4, 91.7 and 93.1 %, respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus heilongjiangensis sp. nov., is proposed and the type strain is S4-3T ( = LMG 26166T = NCIMB 14701T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.053355-0