1887

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, designated isolate J4, was isolated from a neutral saline lake sample from Xinjiang Uyghur Autonomous Region, China, and subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain J4 is most closely related to 25-7 (with 96.7 % similarity), 8-2 (96.5 %), TW25 (96.4 %), CVS-14 (96.4 %) and MB-9 (96.2 %). Chemotaxonomic analysis showed menaquinone-7 (MK-7) to be the major isoprenoid quinone of strain J4; diphosphatidylglycerol and phosphatidylglycerol were the major cellular polar lipids and the cell wall contained -diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were iso-C and anteiso-C. The genomic DNA G+C content of strain J4 was determined to be 36.2 mol%. Strain J4 was positive for catalase activity and negative for oxidase activity. Strain J4 was observed to grow at 25–50 °C (optimal 35–42 °C), pH 6.5–8.0 (optimal 7.0–7.5) and in media containing 1–21 % (w/v) NaCl (optimal 9–12 %). Based on these data, strain J4 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is J4 ( = CGMCC 1.12331 = JCM 18732).

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2014-01-01
2019-10-17
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References

  1. Albuquerque L., Tiago I., Rainey F. A., Taborda M., Nobre M. F., Veríssimo A., da Costa M. S.. ( 2007;). Salirhabdus euzebyi gen. nov., sp. nov., a Gram-positive, halotolerant bacterium isolated from a sea salt evaporation pond. . Int J Syst Evol Microbiol 57:, 1566–1571. [CrossRef][PubMed]
    [Google Scholar]
  2. Amoozegar M. A., Bagheri M., Didari M., Shahzedeh Fazeli S. A., Schumann P., Sánchez-Porro C., Ventosa A.. ( 2013;). Saliterribacillus persicus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a hypersaline lake. . Int J Syst Evol Microbiol 63:, 345–351. [CrossRef][PubMed]
    [Google Scholar]
  3. Ashforth E. J., Fu C.-Z., Liu X.-Y., Dai H.-Q., Song F.-H., Guo H., Zhang L.-X.. ( 2010;). Bioprospecting for antituberculosis leads from microbial metabolites. . Nat Prod Rep 27:, 1709–1719. [CrossRef][PubMed]
    [Google Scholar]
  4. Barrow G. I., Feltham R. K.. ( 1993;). Cowan and Steel's Manual For The Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  5. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A.. ( 2007;). Salsuginibacillus kocurii gen. nov., sp. nov., a moderately halophilic bacterium from soda-lake sediment. . Int J Syst Evol Microbiol 57:, 2381–2386. [CrossRef][PubMed]
    [Google Scholar]
  6. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A.. ( 2008;). Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic, Gram-positive bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 58:, 1961–1967. [CrossRef][PubMed]
    [Google Scholar]
  7. de la Haba R. R., Sánchez-Porro C., Márquez M. C., Ventosa A.. ( 2011;). Taxonomy of halophiles. . In Extremophiles Handbook, pp. 255–308. Edited by Horikoshi K... Tokyo:: Springer;. [CrossRef]
    [Google Scholar]
  8. Didari M., Amoozegar M. A., Bagheri M., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A.. ( 2012;). Alteribacillus bidgolensis gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Bacillus persepolensis as Alteribacillus persepolensis comb. nov.. Int J Syst Evol Microbiol 62:, 2691–2697. [CrossRef][PubMed]
    [Google Scholar]
  9. Echigo A., Minegishi H., Shimane Y., Kamekura M., Usami R.. ( 2012;). Natribacillus halophilus gen. nov., sp. nov., a moderately halophilic and alkalitolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 62:, 289–294. [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. García M. T., Gallego V., Ventosa A., Mellado E.. ( 2005;). Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium. . Int J Syst Evol Microbiol 55:, 1789–1795. [CrossRef][PubMed]
    [Google Scholar]
  12. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A.. ( 1998;). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. . Int J Syst Bacteriol 48:, 99–106. [CrossRef]
    [Google Scholar]
  13. Jeon C. O., Lim J.-M., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J.. ( 2005;). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 55:, 1891–1896. [CrossRef][PubMed]
    [Google Scholar]
  14. Jiang F., Cao S.-J., Li Z.-H., Fan H., Li H.-F., Liu W.-J., Yuan H.-L.. ( 2012;). Salisediminibacterium halotolerans gen. nov., sp. nov., a halophilic bacterium from soda lake sediment. . Int J Syst Evol Microbiol 62:, 2127–2132. [CrossRef][PubMed]
    [Google Scholar]
  15. Kim S. B., Falconer C., Williams E., Goodfellow M.. ( 1998;). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. . Int J Syst Bacteriol 48:, 59–68. [CrossRef][PubMed]
    [Google Scholar]
  16. Kim Y.-G., Hwang C. Y., Yoo K. W., Moon H. T., Yoon J.-H., Cho B. C.. ( 2007;). Pelagibacillus goriensis gen. nov., sp. nov., a moderately halotolerant bacterium isolated from coastal water off the east coast of Korea. . Int J Syst Evol Microbiol 57:, 1554–1560. [CrossRef][PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  18. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  19. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  20. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  21. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J.. ( 2005;). Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. . Int J Syst Evol Microbiol 55:, 165–170. [CrossRef][PubMed]
    [Google Scholar]
  22. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  23. Lu J., Nogi Y., Takami H.. ( 2001;). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. . FEMS Microbiol Lett 205:, 291–297. [CrossRef][PubMed]
    [Google Scholar]
  24. Mandel M., Marmur J.. ( 1968;). Use of ultraviolet absorbance temperature profiles for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  25. Mao J., Wang J., Dai H.-Q., Zhang Z.-D., Tang Q.-Y., Ren B., Yang N., Goodfellow M., Zhang L.-X., Liu Z.-H.. ( 2011;). Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae. . Int J Syst Evol Microbiol 61:, 621–630. [CrossRef][PubMed]
    [Google Scholar]
  26. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  27. Márquez M. C., Carrasco I. J., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A.. ( 2008;). Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. . Int J Syst Evol Microbiol 58:, 1922–1926. [CrossRef][PubMed]
    [Google Scholar]
  28. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  29. Ren P.-G., Zhou P.-J.. ( 2005a;). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. . Int J Syst Evol Microbiol 55:, 95–99. [CrossRef][PubMed]
    [Google Scholar]
  30. Ren P.-G., Zhou P.-J.. ( 2005b;). Salinibacillus aidingensis gen. nov., sp. nov. and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. . Int J Syst Evol Microbiol 55:, 949–953. [CrossRef][PubMed]
    [Google Scholar]
  31. Ren B., Yang N., Wang J., Ma X.-L., Wang Q., Xie F., Guo H., Liu Z.-H., Pugin B., Zhang L.-X.. ( 2013;). Amphibacillus marinus sp. nov., a member of the genus Amphibacillus isolated from marine mud. . Int J Syst Evol Microbiol 63:, 1485–1491. [CrossRef][PubMed]
    [Google Scholar]
  32. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  33. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  34. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M.. ( 2001;). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. . Int J Syst Evol Microbiol 51:, 425–431.[PubMed]
    [Google Scholar]
  35. Simmons J. S.. ( 1926;). A culture medium for differentating organisms of typhoid-colon aerogenes groups and for isolation of certain fungi. . J Infect Dis 39:, 209–214. [CrossRef]
    [Google Scholar]
  36. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K. H.. ( 1996;). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.. Int J Syst Bacteriol 46:, 492–496. [CrossRef]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  38. Tanasupawat S., Namwong S., Kudo T., Itoh T.. ( 2007;). Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand. . Int J Syst Evol Microbiol 57:, 1413–1417. [CrossRef][PubMed]
    [Google Scholar]
  39. Tang Q.-Y., Yang N., Wang J., Xie Y.-Q., Ren B., Zhou Y.-G., Gu M.-Y., Mao J., Li W.-J. et al. ( 2011;). Paenibacillus algorifonticola sp. nov., isolated from a cold spring. . Int J Syst Evol Microbiol 61:, 2167–2172. [CrossRef][PubMed]
    [Google Scholar]
  40. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  41. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A.. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  42. Wainø M., Tindall B. J., Schumann P., Ingvorsen K.. ( 1999;). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:, 821–831. [CrossRef][PubMed]
    [Google Scholar]
  43. Wang X., Xue Y., Ma Y.. ( 2011;). Streptohalobacillus salinus gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil. . Int J Syst Evol Microbiol 61:, 1127–1132. [CrossRef][PubMed]
    [Google Scholar]
  44. Wang Q., Song F.-H., Xiao X., Huang P., Li L., Monte A., Abdel-Mageed W. M., Wang J., Guo H. et al. ( 2013;). Abyssomicins from the South China Sea deep-sea sediment Verrucosispora sp.: natural thioether Michael addition adducts as antitubercular prodrugs. . Angew Chem Int Ed Engl 52:, 1231–1234. [CrossRef][PubMed]
    [Google Scholar]
  45. Xin H., Itoh T., Zhou P., Suzuki K., Nakase T.. ( 2001;). Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. . Int J Syst Evol Microbiol 51:, 1825–1829. [CrossRef][PubMed]
    [Google Scholar]
  46. Yoon J.-H., Kang K.-H., Park Y.-H.. ( 2002;). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. . Int J Syst Evol Microbiol 52:, 2043–2048. [CrossRef][PubMed]
    [Google Scholar]
  47. Yoon J.-H., Kang S.-J., Oh T.-K.. ( 2007;). Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov.. Int J Syst Evol Microbiol 57:, 2406–2411. [CrossRef][PubMed]
    [Google Scholar]
  48. Zhang L.-X., Yan K.-Z., Zhang Y., Huang R., Bian J., Zheng C.-S., Sun H.-X., Chen Z.-H., Sun N. et al. ( 2007;). High-throughput synergy screening identifies microbial metabolites as combination agents for the treatment of fungal infections. . Proc Natl Acad Sci U S A 104:, 4606–4611. [CrossRef][PubMed]
    [Google Scholar]
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