@article{mbs:/content/journal/ijsem/10.1099/ijs.0.053272-0, author = "Hamada, Moriyuki and Shibata, Chiyo and Tamura, Tomohiko and Suzuki, Ken-ichiro", title = "Zhihengliuella flava sp. nov., an actinobacterium isolated from sea sediment, and emended description of the genus Zhihengliuella", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_12", pages = "4760-4764", doi = "https://doi.org/10.1099/ijs.0.053272-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.053272-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel Gram-staining-positive actinobacterium, designated H85-3T, was isolated from a sea sediment sample and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain H85-3T was closely related to the members of the genus Zhihengliuella with pairwise sequence similarities of 97.4–98.6 %. The peptidoglycan of strain H85-3T was found to be of the A4α type with lysine as the diagnostic diamino acid. The menaquinones were MK-9, MK-10 and MK-8 (56 : 30 : 14) and the major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. These data supported the affiliation of strain H85-3T to the genus Zhihengliuella . Meanwhile, the results of DNA–DNA hybridization, along with the differences in some phenotypic characteristics, indicated that strain H85-3T should be distinguished from the recognized species of the genus Zhihengliuella . Therefore, strain H85-3T represents a novel species of the genus Zhihengliuella , for which the name Zhihengliuella flava sp. nov. is proposed; the type strain is H85-3T ( = NBRC 109021T = DSM 26152T). An emended description of the genus Zhihengliuella is also proposed.", }