1887

Abstract

Two thermophilic bacteria, designated strains P8 and P12, were isolated from compost in Korea. The isolates grew aerobically from 50 to 75 °C (optimum at 55 °C) and at pH 4.0–9.0 (optimum pH 6.5). Aerial mycelia were not observed. Single spores were produced along the substrate hypha. The predominant menaquinone was MK-7. Major fatty acids were iso-C, iso-C and iso-C. The cell wall contained -diaminopimelic acid and the polar lipids were phosphatidylethanolamine, an aminophospholipid and sphingoglycolipid. The DNA G+C contents were 55.9–56.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains P8 and P12 belonged to the genus in the family with sequence similarities of 96.1–97.2 %. Levels of DNA–DNA relatedness between strain P8 and the type strains of recognized species of the genus ranged from 28.9 to 38.2 %. On the basis of data from the present polyphasic study, strains P8 and P12 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P8 ( = KACC 16581 = NBRC 108858).

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2013-12-01
2019-12-05
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