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Volume 63, Issue Pt_9

Morphology and small-subunit rRNA gene sequences of two novel marine ciliates, spec. nov. and spec. nov. (Protista, Ciliophora, Scuticociliatia), with an improved diagnosis of the genus Free

Abstract

The morphology and infraciliature of two novel marine scuticociliates, spec. nov. and spec. nov., collected from sandy beaches at Qingdao, China, were investigated using live observation and protargol-staining methods. spec. nov. is distinguished by the following characteristics: marine habitat and a slender to elongate oval body with pointed anterior end and rounded caudal end, about 25–50 µm long; buccal field about a quarter to a third of body length; nine or ten somatic kineties with dikinetids approximately in anterior half of body, monokinetids in posterior half; membranelles 1 and 2 almost equal in length and composed of two and three longitudinal rows of kinetids respectively; paroral membrane with zigzag structure extending anteriorly to middle portion of membranelle 2; contractile vacuole pore located at posterior end of somatic kinety 1. The genus is redefined as follows: marine form with an elongate-elliptical or inverted pear-shaped body; apical plate conspicuous; buccal field about two-thirds of body length, cytostome subequatorially located; oral apparatus -like; somatic kineties comprising a mixture of dikinetids and monokinetids. spec. nov. is recognized by having an elongate-elliptical body with truncated apical frontal plate, size about 25–35×15–20 µm, nine or ten somatic kineties, membranelle 1 consisting of two or three basal bodies, contractile vacuole pore at posterior end of somatic kinety 1. This study also compared the small-subunit rRNA gene sequences of these two species with other closely related species to show the sequence divergence, which ranged from 3.53 to 9.60 %. Phylogenetic analyses support the contention that the genus is monophyletic, while is non-monophyletic.

  • Published Online:
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31272285 and 41176119)
  • Marie Curie International Incoming Fellowship within the 7th European Community Framework Programme (Award 909381)
  • Research Group Project from King Saud University Deanship of Scientific Research, Saudi Arabia (Award RGP-VPP-083)
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2013-09-01
2024-04-18
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Morphology and small-subunit rRNA gene sequences of two novel marine ciliates, Metanophrys orientalis spec. nov. and Uronemella sinensis spec. nov. (Protista, Ciliophora, Scuticociliatia), with an improved diagnosis of the genus Uronemella
Int J Syst Evol Microbiol 63, 3515 (2013); https://doi.org/10.1099/ijs.0.053173-0
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