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Abstract

A strain of Gram-staining-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacteria, designated WS-MY3, was isolated from a brown algae reservoir in South Korea. Strain WS-MY3 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain WS-MY3 fell within the cluster comprising the type strains of species of the genus , clustering coherently with the type strains of and . It exhibited 16S rRNA gene sequence similarity values of 98.7, 97.9 and 96.8 % to the type strains of . , . and , respectively. Strain WS-MY3 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH, iso-C G and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain WS-MY3 were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain WS-MY3 was 37.3 mol% and its DNA–DNA relatedness values with . KCTC 12365 and . KCTC 12364 were 23 % and 17 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WS-MY3 is separate from the three recognized species of the genus . On the basis of the data presented, strain WS-MY3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WS-MY3 ( = KCTC 32328 = CECT 8286).

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2013-11-01
2019-10-23
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