1887

Abstract

A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain WM35, was isolated from the intestinal tract of an abalone, , which was collected from the northern coast of Jeju in Korea. The cells of the isolate grew optimally at 30 °C, pH 7, and with 3 % (w/v) NaCl. Based on 16S rRNA gene sequence similarity comparisons, strain WM35 was grouped in the genus and was closely related to the type strains of (98.7 % similarity) and (97.8 % similarity). The major cellular fatty acids were summed feature 8 and C 2-OH. Ubiquinone Q-10 was the predominant respiratory quinone. The polar lipids of strain WM35 comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of the isolate was 53.8 mol%. DNA–DNA hybridization values indicated <16 % genomic relatedness with members of the genus . The physiological, chemical and genotypic analyses indicated that strain WM35 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WM35 ( = KACC 17212 = JCM 18870).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053140-0
2013-11-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4248.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053140-0&mimeType=html&fmt=ahah

References

  1. Bae J. W. , Rhee S. K. , Park J. R. , Chung W. H. , Nam Y. D. , Lee I. , Kim H. , Park Y. H. . ( 2005; ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71:, 8825–8835. [CrossRef] [PubMed]
    [Google Scholar]
  2. Benson H. J. . ( 1994; ). Microbiological Applications: a Laboratory Manual in General Microbiology, , 6th edn.. Dubuque, IA:: Wm. C. Brown;.
    [Google Scholar]
  3. Chang H. W. , Nam Y. D. , Jung M. Y. , Kim K. H. , Roh S. W. , Kim M. S. , Jeon C. O. , Yoon J. H. , Bae J. W. . ( 2008; ). Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75:, 523–530. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chen M. H. , Sheu S. Y. , Chen C. A. , Wang J. T. , Chen W. M. . ( 2011; ). Shimia isoporae sp. nov., isolated from the reef-building coral Isopora palifera . . Int J Syst Evol Microbiol 61:, 823–827. [CrossRef] [PubMed]
    [Google Scholar]
  5. Choi D. H. , Cho B. C. . ( 2006; ). Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. . Int J Syst Evol Microbiol 56:, 1869–1873. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. , Jones D. . ( 1981a; ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45:, 316–354.[PubMed]
    [Google Scholar]
  7. Collins M. D. , Jones D. . ( 1981b; ). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51:, 129–134. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef] [PubMed]
    [Google Scholar]
  10. Goszczynska T. , Serfontein J. J. . ( 1998; ). Milk-Tween agar, a semiselective medium for isolation and differentiation of Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv. phaseoli. . J Microbiol Methods 32:, 65–72. [CrossRef]
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  13. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . New York:: Wiley;.
    [Google Scholar]
  14. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, Version 3.0. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  15. Oakes F. R. , Ponte R. D. . ( 1996; ). The abalone market: opportunities for cultured abalone. . Aquaculture 140:, 187–195. [CrossRef]
    [Google Scholar]
  16. Rochelle P. A. , Fry J. C. , Parkes R. J. , Weightman A. J. . ( 1992; ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 79:, 59–65.[PubMed] [CrossRef]
    [Google Scholar]
  17. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  19. Sawabe T. , Oda Y. , Shiomi Y. , Ezura Y. . ( 1995; ). Alginate degradation by bacteria isolated from the gut of sea-urchins and abalones. . Microb Ecol 30:, 193–202. [CrossRef]
    [Google Scholar]
  20. Sawabe T. , Inoue S. , Fukui Y. , Yoshie K. , Nishihara Y. , Miura H. . ( 2007; ). Mass mortality of Japanese abalone Haliotis discus hannai caused by Vibrio harveyi infection. . Microbes Environ 22:, 300–308. [CrossRef]
    [Google Scholar]
  21. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tanaka R. , Sugimura I. , Sawabe T. , Yoshimizu M. , Ezura Y. . ( 2003; ). Gut microflora of abalone Haliotis discus hannai in culture changes coincident with a change in diet. . Fish Sci 69:, 951–958. [CrossRef]
    [Google Scholar]
  23. Tanaka R. , Ootsubo M. , Sawabe T. , Ezura Y. , Tajima K. . ( 2004; ). Biodiversity and in situ abundance of gut microflora of abalone (Haliotis discus hannai) determined by culture-independent techniques. . Aquaculture 241:, 453–463. [CrossRef]
    [Google Scholar]
  24. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tindall B. J. . ( 1990; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  26. Tittsler R. P. , Sandholzer L. A. . ( 1936; ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  27. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.053140-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053140-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error